LOCUS       BBa_I12037              1290 bp    DNA     linear       10-May-2024
DEFINITION  Reporter 3 for Barkai-Leibler oscillator
ACCESSION   BBa_I12037
VERSION     BBa_I12037.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    146..162
                     /label=OR1 lambda
     misc_feature    170..186
                     /label=OR2 lambda
     misc_feature    193..206
                     /label=OR1 434
     misc_feature    215..228
                     /label=OR2 434
     promoter        185..190
                     /label=-35
     promoter        208..213
                     /label=-10
     misc_feature    220..220
                     /label=start
     promoter        138..228
                     /label=BBa_I12036
     misc_feature    241..244
                     /label=conserved
     RBS             237..248
                     /label=BBa_B0034
     CDS             255..1016
                     /label=YFP (LVA)
     misc_feature    1011..1016
                     /label=2
     misc_feature    972..1010
                     /label=SsrA
     misc_feature    496..496
                     /label=A (Q->L)
     misc_feature    255..1016
                     /label=BBa_E0032
     misc_feature    255..1016
                     /label=BBa_E0032
     stem_loop       1036..1079
                     /label=stem_loop
     misc_feature    1025..1104
                     /label=BBa_B0010
     terminator      1025..1104
                     /label=BBa_B0010
     stem_loop       1120..1139
                     /label=T7 TE
     misc_feature    1146..1146
                     /label=stop
     polyA_site      1140..1153
                     /label=polya
     misc_feature    1113..1153
                     /label=BBa_B0012
     terminator      1113..1153
                     /label=BBa_B0012
     misc_feature    237..1153
                     /label=BBa_E0432
     stem_loop       1173..1216
                     /label=stem_loop
     misc_feature    1162..1241
                     /label=BBa_B0010
     terminator      1162..1241
                     /label=BBa_B0010
     stem_loop       1257..1276
                     /label=T7 TE
     misc_feature    1283..1283
                     /label=stop
     polyA_site      1277..1290
                     /label=polya
     misc_feature    1250..1290
                     /label=BBa_B0012
     terminator      1250..1290
                     /label=BBa_B0012
     terminator      1162..1290
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
      181 gtgttagata ttacaaactt tcttgtatag atttacaatg tatcttgtta ctagagaaag
      241 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      301 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      361 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      421 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
      481 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      541 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      601 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      661 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      721 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      781 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      841 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
      901 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      961 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact
     1021 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1081 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1141 ttctgcgttt atatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1201 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1261 caccttcggg tgggcctttc tgcgtttata
//