LOCUS BBa_I12042 4128 bp DNA linear 13-May-2024 DEFINITION BL oscillator, no cooperativity, reporter protein, kickstart ACCESSION BBa_I12042 VERSION BBa_I12042.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..830 /label=BBa_C0051 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 misc_feature 1..992 /label=BBa_I12038 misc_feature 1001..1041 /label=B0012 misc_feature 1050..1129 /label=B0010 terminator 1001..1129 /label=BBa_B0025 misc_feature 1193..1206 /label=OR1 434 misc_feature 1146..1162 /label=OR1 lambda misc_feature 1170..1186 /label=OR2 lambda promoter 1208..1213 /label=-10 promoter 1185..1190 /label=-35 promoter 1138..1219 /label=BBa_I12006 misc_feature 1232..1235 /label=conserved RBS 1228..1239 /label=BBa_B0034 CDS 1249..1956 /label=cI lambda misc_feature 1957..1989 /label=LVA misc_feature 1996..2020 /label=Help:Barcodes CDS 1246..2020 /label=BBa_C0051 misc_feature 2033..2036 /label=conserved RBS 2029..2040 /label=BBa_B0034 misc_feature 2053..2056 /label=conserved RBS 2049..2060 /label=BBa_B0034 CDS 2067..2828 /label=YFP (LVA) misc_feature 2823..2828 /label=2 misc_feature 2784..2822 /label=SsrA misc_feature 2308..2308 /label=A (Q->L) misc_feature 2067..2828 /label=BBa_E0032 misc_feature 2067..2828 /label=BBa_E0032 stem_loop 2848..2891 /label=stem_loop misc_feature 2837..2916 /label=BBa_B0010 terminator 2837..2916 /label=BBa_B0010 stem_loop 2932..2951 /label=T7 TE misc_feature 2958..2958 /label=stop polyA_site 2952..2965 /label=polya misc_feature 2925..2965 /label=BBa_B0012 terminator 2925..2965 /label=BBa_B0012 misc_feature 2049..2965 /label=BBa_E0432 stem_loop 2985..3028 /label=stem_loop misc_feature 2974..3053 /label=BBa_B0010 terminator 2974..3053 /label=BBa_B0010 stem_loop 3069..3088 /label=T7 TE misc_feature 3095..3095 /label=stop polyA_site 3089..3102 /label=polya misc_feature 3062..3102 /label=BBa_B0012 terminator 3062..3102 /label=BBa_B0012 terminator 2974..3102 /label=BBa_B0015 CDS 1001..3102 /label=BBa_I12043 misc_feature 3111..3151 /label=B0012 misc_feature 3160..3239 /label=B0010 terminator 3111..3239 /label=BBa_B0025 misc_feature 3256..3272 /label=OR1 misc_feature 3280..3296 /label=OR2 promoter 3295..3300 /label=-35 misc_feature 3306..3312 /label=mutate to obliterate OR3 promoter 3318..3323 /label=-10 promoter 3248..3329 /label=BBa_I12007 misc_feature 3342..3345 /label=conserved RBS 3338..3349 /label=BBa_B0034 CDS 3356..4024 /label=cI 434 promoter 3356..3991 /label=BBa_I12014 stem_loop 4011..4054 /label=stem_loop misc_feature 4000..4079 /label=BBa_B0010 terminator 4000..4079 /label=BBa_B0010 stem_loop 4095..4114 /label=T7 TE misc_feature 4121..4121 /label=stop polyA_site 4115..4128 /label=polya misc_feature 4088..4128 /label=BBa_B0012 terminator 4088..4128 /label=BBa_B0012 terminator 4000..4128 /label=BBa_B0015 misc_feature 3111..4128 /label=BBa_I12002 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tataaacgca gaaaggccca 1021 cccgaaggtg agccagtgtg actctagtag agagcgttca ccgacaaaca acagataaaa 1081 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggt actagaggca 1141 accattatca ccgccagagg taaaatagtc aacacgcacg gtgttagata ttacaaactt 1201 tcttgtatag atttaacgtt actagagaaa gaggagaaat actagatgag cacaaaaaag 1261 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa 1321 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag 1381 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg 1441 cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc 1501 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct 1561 gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt 1621 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag 1681 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg 1741 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt 1801 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa 1861 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa 1921 gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga aaactacgct 1981 ttagtagctt aataacgctg atagtgctag tgtagatcgc tactagagaa agaggagaaa 2041 tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg 2101 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 2161 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 2221 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct 2281 tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag 2341 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 2401 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 2461 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 2521 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 2581 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 2641 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc 2701 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 2761 tcggcatgga cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctttagtag 2821 cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2881 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2941 tcgggtgggc ctttctgcgt ttatatacta gagccaggca tcaaataaaa cgaaaggctc 3001 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3061 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tataaacgca 3121 gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca ccgacaaaca 3181 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggt 3241 actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg gtgttagata 3301 tttataaata gtggtgatag atttaacgtt actagagaaa gaggagaaat actagatgag 3361 tatttcttcc agggtaaaaa gcaaaagaat ccagcttgga cttaaccagg ctgaacttgc 3421 tcaaaaggtg gggactaccc agcagtctat agagcagctc gaaaacggta aaactaagcg 3481 accacgcttt ttaccagaac ttgcgtcagc tcttggcgta agtgttgact ggctgctcaa 3541 tggcacctct gattcgaatg ttagatttgt tgggcacgtt gagcccaaag ggaaatatcc 3601 attgattagc atggttagag ctggttcgtg gtgtgaagct tgtgaaccct acgatatcaa 3661 ggacattgat gaatggtatg acagtgacgt taacttatta ggcaatggat tctggctgaa 3721 ggttgaaggt gattccatga cctcacctgt aggtcaaagc atccctgaag gtcatatggt 3781 gttagtagat actggacggg agccagtgaa tggaagcctt gttgtagcca aactgactga 3841 cgcgaacgaa gcaacattca agaaactggt catagatggc ggtcagaagt acctgaaagg 3901 cctgaatcct tcatggccta tgactcctat caacggaaac tgcaagatta tcggtgttgt 3961 cgtggaagcg agggtaaaat tcgtataata atactagagc caggcatcaa ataaaacgaa 4021 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 4081 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //