LOCUS       BBa_I12057              1128 bp    DNA     linear       08-May-2024
DEFINITION  A protein (lambda cI) subassembly for modified BL oscillator based 
            on I12040
ACCESSION   BBa_I12057
VERSION     BBa_I12057.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1 lambda
     misc_feature    33..49
                     /label=OR2 lambda
     promoter        48..53
                     /label=-35
     misc_feature    56..69
                     /label=OR1 434
     promoter        71..76
                     /label=-10
     misc_feature    78..91
                     /label=OR2 434
     misc_feature    83..83
                     /label=start
     promoter        1..91
                     /label=BBa_I12040
     misc_feature    104..107
                     /label=conserved
     RBS             100..111
                     /label=BBa_B0034
     CDS             121..828
                     /label=cI lambda
     misc_feature    829..861
                     /label=LVA
     misc_feature    868..892
                     /label=Help:Barcodes
     CDS             118..867
                     /label=BBa_C0051
     stem_loop       912..955
                     /label=stem_loop
     misc_feature    901..980
                     /label=BBa_B0010
     terminator      901..980
                     /label=BBa_B0010
     stem_loop       996..1015
                     /label=T7 TE
     misc_feature    1022..1022
                     /label=stop
     polyA_site      1016..1029
                     /label=polya
     misc_feature    989..1029
                     /label=BBa_B0012
     terminator      989..1029
                     /label=BBa_B0012
     misc_feature    1046..1062
                     /label=OR1 lambda
     misc_feature    1070..1086
                     /label=OR2 lambda
     promoter        1085..1090
                     /label=-35
     misc_feature    1093..1106
                     /label=OR1 434
     promoter        1108..1113
                     /label=-10
     misc_feature    1115..1128
                     /label=OR2 434
     misc_feature    1120..1120
                     /label=start
     promoter        1038..1128
                     /label=BBa_I12040
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atattacaaa
       61 ctttcttgta gatacttaca atgtatcttg ttactagaga aagaggagaa atactagatg
      121 agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg ccttaaagca
      181 atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg
      241 gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt aaatgcttat
      301 aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag cccttcaatc
      361 gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact tagaagtgag
      421 tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga gcttagaacc
      481 tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca
      541 ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa gccaagcttt
      601 cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg tgatttctgc
      661 atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga tagcggtcag
      721 gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga gagttgttcc
      781 gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac
      841 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag
      901 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      961 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1021 gcgtttatat actagaggca accattatca ccgccagagg taaaatagtc aacacgcacg
     1081 gtgttagata ttacaaactt tcttgtagat acttacaatg tatcttgt
//