Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: rwald
Date created: 2004-08-05 11:00:00
Date modified: 2015-08-31 04:07:31
TetR - TetR-4C heterodimer promoter (negative)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_I12212_sequence (Version 1) |
Description
This promoter is repressed by a heterodimer of wild-type TetR and mutant TetR-4C. It differs from wild-type TetR promoter in that the +4 and -4 regions of its O2 binding site have been changed, and the -35 region of the reverse promoter (pA) has been destroyed.Notes
The O2 and O1 mutations are all single point mutations, occuring at the first base which appears listed here.| Sequence Annotation | Location | Component / Role(s) |
| -35 TetR O2 -35 -10 O1 -10 O2 mutation O1 mutation | 1,6 2,20 22,27 26,30 32,50 45,50 7,7 37,37 | feature/promoter promoter feature/binding non_covalent_binding_site feature/promoter promoter feature/promoter promoter feature/binding non_covalent_binding_site feature/promoter promoter non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding |