LOCUS       BBa_I12220              1018 bp    DNA     linear       06-May-2024
DEFINITION  Lambda cI - Gal4 fusion protein generator
ACCESSION   BBa_I12220
VERSION     BBa_I12220.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..730
                     /label=cI lambda
     CDS             731..850
                     /label=gal4 dimerization domain
     stem_loop       851..856
                     /label=Terminator
     CDS             20..853
                     /label=cI lambda
     misc_feature    857..881
                     /label=Help:Barcodes
     CDS             20..856
                     /label=BBa_I12200
     stem_loop       901..944
                     /label=stem_loop
     misc_feature    890..969
                     /label=BBa_B0010
     terminator      890..969
                     /label=BBa_B0010
     stem_loop       985..1004
                     /label=T7 TE
     misc_feature    1011..1011
                     /label=stop
     polyA_site      1005..1018
                     /label=polya
     misc_feature    978..1018
                     /label=BBa_B0012
     terminator      978..1018
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga
       61 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca
      121 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg
      181 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt
      241 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat
      301 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat
      361 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac
      421 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc
      481 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc
      541 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa
      601 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat
      661 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga
      721 gacgtttggc gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
      781 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
      841 aggattattt taataaccct gatagtgcta gtgtagatcc ctactagagc caggcatcaa
      901 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      961 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//