LOCUS       BBa_I12221              1201 bp    DNA     linear       04-May-2024
DEFINITION  Alpha-NTD - Gal11p fusion protein generator
ACCESSION   BBa_I12221
VERSION     BBa_I12221.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..724
                     /label=Alpha N-terminal domain
     misc_feature    725..763
                     /label=Flexible linker
     CDS             764..1033
                     /label=Gal11p dimerization domain
     stem_loop       1034..1039
                     /label=Terminator
     CDS             20..1036
                     /label=NTD - Gal Alpha
     misc_feature    1040..1064
                     /label=Help:Barcodes
     CDS             20..1039
                     /label=BBa_I12201
     stem_loop       1084..1127
                     /label=stem_loop
     misc_feature    1073..1152
                     /label=BBa_B0010
     terminator      1073..1152
                     /label=BBa_B0010
     stem_loop       1168..1187
                     /label=T7 TE
     misc_feature    1194..1194
                     /label=stop
     polyA_site      1188..1201
                     /label=polya
     misc_feature    1161..1201
                     /label=BBa_B0012
     terminator      1161..1201
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgcagggttc tgtgacagag tttctaaaac cgcgcctggt
       61 tgatatcgag caagtgagtt cgacgcacgc caaggtgacc cttgagcctt tagagcgtgg
      121 ctttggccat actctgggta acgcactgcg ccgtattctg ctctcatcga tgccgggttg
      181 cgcggtgacc gaggttgaga ttgatggtgt actacatgag tacagcacca aagaaggcgt
      241 tcaggaagat atcctggaaa tcctgctcaa cctgaaaggg ctggcggtga gagttcaggg
      301 caaagatgaa gttattctta ccttgaataa atctggcatt ggccctgtga cggcagccga
      361 tatcacccac gacggtgatg tcgaaatcgt caagccgcag cacgtgatct gccacctgac
      421 cgatgagaac gcgtctatta gcatgcgtat caaagttcag cgcggtcgtg gttatgtgcc
      481 ggcttctacc cgaatccatt cggaagaaga tgagcgccca atcggccgtc tgctggtcga
      541 cgcatgctac agccctgtgg agcgtattgc ctacaatgtt gaagcagcgc gtgtagaaca
      601 gcgtaccgac ctggacaagc tggtcatcga aatggaaacc aacggcacaa tcgatcctga
      661 agaggcgatt cgtcgtgcgg caaccattct ggctgaacaa ctggaagctt tcgttgactt
      721 acgtgatgta cgtcagcctg aagtgaaaga agagaaacca gagcctcaac agcagcaaat
      781 gcaacctccc aattcaagtg cgaacaacaa ccctttgcaa cagcaatcat cacaaaatac
      841 cgtaccaaac gtcctcaacc aaattaacca aatcttttct ccagaggagc aacgcagctt
      901 attacaagaa gccatcgaaa cctgcaagaa ttttgaaaaa acacaattgg gtagtacgat
      961 gacggaacct gtcaagcaaa gttttattag gaaatacatt aatcaaaagg ccctgagaaa
     1021 aatccaagct ttgtaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
     1081 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1141 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1201 a
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