LOCUS       BBa_I12222               808 bp    DNA     linear       06-May-2024
DEFINITION  Wild-type TetR(B) protein generator
ACCESSION   BBa_I12222
VERSION     BBa_I12222.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..646
                     /label=tetR
     misc_feature    647..671
                     /label=Help:Barcodes
     CDS             20..646
                     /label=BBa_I12202
     stem_loop       691..734
                     /label=stem_loop
     misc_feature    680..759
                     /label=BBa_B0010
     terminator      680..759
                     /label=BBa_B0010
     stem_loop       775..794
                     /label=T7 TE
     misc_feature    801..801
                     /label=stop
     polyA_site      795..808
                     /label=polya
     misc_feature    768..808
                     /label=BBa_B0012
     terminator      768..808
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
       61 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      121 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
      181 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      241 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      301 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
      361 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
      421 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
      481 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
      541 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
      601 atgcggatta gaaaaacaac ttaaatgtga aagtgggtct taataactct gatagtgcta
      661 gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      721 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      781 ccttcgggtg ggcctttctg cgtttata
//