LOCUS       BBa_I12224              1267 bp    DNA     linear       02-May-2024
DEFINITION  LacI protein generator (LVA-)
ACCESSION   BBa_I12224
VERSION     BBa_I12224.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    1106..1130
                     /label=Help:Barcodes
     CDS             20..1105
                     /label=BBa_C0013
     stem_loop       1150..1193
                     /label=stem_loop
     misc_feature    1139..1218
                     /label=BBa_B0010
     terminator      1139..1218
                     /label=BBa_B0010
     stem_loop       1234..1253
                     /label=T7 TE
     misc_feature    1260..1260
                     /label=stop
     polyA_site      1254..1267
                     /label=polya
     misc_feature    1227..1267
                     /label=BBa_B0012
     terminator      1227..1267
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
       61 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
      121 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
      181 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
      241 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
      301 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
      361 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
      421 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
      481 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
      541 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
      601 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
      661 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
      721 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
      781 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
      841 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
      901 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
      961 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     1021 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     1081 ccgactggaa agcgggcagt aataacactg atagtgctag tgtagatcac tactagagcc
     1141 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1201 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1261 gtttata
//