LOCUS BBa_I12233 6513 bp DNA linear 19-May-2024 DEFINITION TetR heterodimer test construct 2 ACCESSION BBa_I12233 VERSION BBa_I12233.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=OR3 misc_feature 11..28 /label=OR2 misc_feature 34..51 /label=OR1 promoter 18..23 /label=-35 promoter 8..13 /label=-35 promoter 30..35 /label=-10 misc_feature 1..54 /label=BBa_R0053 promoter 1..54 /label=BBa_R0053 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 misc_feature 1168..1192 /label=Help:Barcodes CDS 82..1167 /label=BBa_C0013 stem_loop 1212..1255 /label=stem_loop misc_feature 1201..1280 /label=BBa_B0010 terminator 1201..1280 /label=BBa_B0010 stem_loop 1296..1315 /label=T7 TE misc_feature 1322..1322 /label=stop polyA_site 1316..1329 /label=polya misc_feature 1289..1329 /label=BBa_B0012 terminator 1289..1329 /label=BBa_B0012 misc_feature 1338..1340 /label=OR3 misc_feature 1348..1365 /label=OR2 misc_feature 1371..1388 /label=OR1 promoter 1355..1360 /label=-35 promoter 1345..1350 /label=-35 promoter 1367..1372 /label=-10 misc_feature 1338..1391 /label=BBa_R0053 promoter 1338..1391 /label=BBa_R0053 RBS 1400..1412 /label=RBS-3\Weak RBS 1406..1409 /label=RBS misc_feature 1400..1412 /label=BBa_B0032 RBS 1400..1412 /label=BBa_B0032 CDS 1419..2300 /label=araC misc_feature 2301..2325 /label=Help:Barcodes CDS 1419..2300 /label=BBa_I12205 stem_loop 2345..2388 /label=stem_loop misc_feature 2334..2413 /label=BBa_B0010 terminator 2334..2413 /label=BBa_B0010 stem_loop 2429..2448 /label=T7 TE misc_feature 2455..2455 /label=stop polyA_site 2449..2462 /label=polya misc_feature 2422..2462 /label=BBa_B0012 terminator 2422..2462 /label=BBa_B0012 CDS 2471..2558 /label=end of LacI coding region (inactive) misc_feature 2471..2670 /label=BBa_R0010 misc_feature 2559..2596 /label=CAP binding site promoter 2607..2612 /label=-35 promoter 2631..2636 /label=-10 misc_feature 2636..2670 /label=LacI binding site misc_feature 2643..2643 /label=start promoter 2471..2670 /label=BBa_R0010 RBS 2679..2691 /label=RBS-3\Weak RBS 2685..2688 /label=RBS misc_feature 2679..2691 /label=BBa_B0032 RBS 2679..2691 /label=BBa_B0032 CDS 2698..3324 /label=tetR misc_feature 3325..3349 /label=Help:Barcodes CDS 2698..3324 /label=BBa_I12202 stem_loop 3369..3412 /label=stem_loop misc_feature 3358..3437 /label=BBa_B0010 terminator 3358..3437 /label=BBa_B0010 stem_loop 3453..3472 /label=T7 TE misc_feature 3479..3479 /label=stop polyA_site 3473..3486 /label=polya misc_feature 3446..3486 /label=BBa_B0012 terminator 3446..3486 /label=BBa_B0012 promoter 4574..4704 /label=Pbad promoter 4537..4608 /label=PC misc_feature 3495..4573 /label=AraC promoter 3495..4704 /label=BBa_I0500 misc_feature 4717..4720 /label=conserved RBS 4713..4724 /label=BBa_B0034 CDS 4731..5357 /label=tetR-4C misc_feature 5358..5382 /label=Help:Barcodes CDS 4731..5357 /label=BBa_I12203 stem_loop 5402..5445 /label=stem_loop misc_feature 5391..5470 /label=BBa_B0010 terminator 5391..5470 /label=BBa_B0010 stem_loop 5486..5505 /label=T7 TE misc_feature 5512..5512 /label=stop polyA_site 5506..5519 /label=polya misc_feature 5479..5519 /label=BBa_B0012 terminator 5479..5519 /label=BBa_B0012 promoter 5528..5533 /label=-35 misc_feature 5529..5547 /label=TetR O2 promoter 5549..5554 /label=-35 promoter 5553..5557 /label=-10 misc_feature 5559..5577 /label=O1 promoter 5572..5577 /label=-10 misc_feature 5534..5534 /label=O2 mutation misc_feature 5564..5564 /label=O1 mutation promoter 5528..5588 /label=BBa_I12212 misc_feature 5601..5604 /label=conserved RBS 5597..5608 /label=BBa_B0034 CDS 5615..6376 /label=CFP (LVA) misc_feature 6333..6370 /label=SsrA misc_feature 6371..6376 /label=2 misc_feature 5683..5683 /label=A misc_feature 5615..6376 /label=BBa_E0022 misc_feature 5615..6376 /label=BBa_E0022 stem_loop 6396..6439 /label=stem_loop misc_feature 6385..6464 /label=BBa_B0010 terminator 6385..6464 /label=BBa_B0010 stem_loop 6480..6499 /label=T7 TE misc_feature 6506..6506 /label=stop polyA_site 6500..6513 /label=polya misc_feature 6473..6513 /label=BBa_B0012 terminator 6473..6513 /label=BBa_B0012 ORIGIN 1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag 61 agtcacacag gaaagtacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc 121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1141 tcccgactgg aaagcgggca gtaataacac tgatagtgct agtgtagatc actactagag 1201 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1261 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1321 gcgtttatat actagagaat aaacttgact aaagattcct ttagtagata atttaagtgt 1381 tctttaattt ctactagagt cacacaggaa agtactagat ggctgaagcg caaaatgatc 1441 ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg ccgattgagg 1501 ccaacggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt tatattctca 1561 atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt gtctgccgac 1621 cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt catccggagg 1681 ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg catgaatggc 1741 ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa gcgcaccagc 1801 cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa gggcgctatt 1861 cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg gaagcgatta 1921 acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag tacatcagcg 1981 atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt tgcttgtcgc 2041 cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta agctggcgcg 2101 aggaccaacg cattagtcag gcgaagctgc ttttgagcac tacccggatg cctatcgcca 2161 ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta tttaaaaaat 2221 gcaccggggc cagcccgagc gagtttcgtg ccggttgtga agaaaaagtg aatgatgtag 2281 ccgtcaagtt gtcataataa cgctgatagt gctagtgtag atcgctacta gagccaggca 2341 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 2401 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 2461 tatactagag caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 2521 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 2581 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 2641 ggaattgtga gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg 2701 tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga 2761 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg 2821 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat 2881 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat 2941 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta 3001 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc 3061 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact 3121 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct 3181 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt 3241 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt 3301 aaatgtgaaa gtgggtctta ataactctga tagtgctagt gtagatctct actagagcca 3361 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3421 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3481 tttatatact agagttatga caacttgacg gctacatcat tcactttttc ttcacaaccg 3541 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt 3601 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa 3661 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac 3721 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg 3781 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc 3841 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat 3901 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta 3961 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac 4021 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga 4081 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc 4141 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt 4201 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt 4261 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga 4321 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc 4381 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac 4441 tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt 4501 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac 4561 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt 4621 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct 4681 ccatacccgt ttttttgggc tagctactag agaaagagga gaaatactag atgtccagat 4741 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 4801 gtttaacaac ccgtaaactc gcccagaagc taggtgtagc gcagcaaaca ttgatgtggc 4861 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 4921 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 4981 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 5041 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 5101 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 5161 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 5221 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 5281 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 5341 aaagtgggtc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca 5401 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 5461 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 5521 actagagttc tctgtcactg atagggagtg gtaaaataac tctgtcaatg atagagtgga 5581 ttcaaaaata ctagagaaag aggagaaata ctagatggtg agcaagggcg aggagctgtt 5641 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtgaacggcc acaagttcag 5701 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 5761 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt 5821 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 5881 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 5941 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 6001 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca 6061 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg 6121 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 6181 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 6241 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 6301 gatcactctc ggcatggacg agctgtacaa gaggcctgct gcaaacgacg aaaactacgc 6361 tttagtagct taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 6421 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 6481 gctcaccttc gggtgggcct ttctgcgttt ata //