BBa_I13015

BBa_I13015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I13015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Debra, Fred, Jay, Madeleine, Cell-Cell Signaling SMUG

Date created: 2004-07-07 11:00:00

Date modified: 2015-08-31 04:07:32

3OC₆HSL Sender controlled by pBad

Details

• Types: DnaRegion
• Roles: Signalling; engineered_region
• Sequences: BBa_I13015_sequence (Version 1)

Description

The pBad promoter controlling a LuxI sender device. The protein is an untagged LuxI enzyme which produces an AHL (3OC₆HSL).

Notes

The pictures below are from a time course, where pRCV-3 produced GFP via HSL-bound LuxR promotion. I13015 was on the left half of the slide (induced in .1% arabinose, thereby producing the HSL for pRCV-3), while pRCV-3 was on the right half.

Controls were done with pRCV-3 alone, I13015 alone and the two together without any arabinose. In all three cases little or no GFP was expressed.

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_B0034	BBa_B0034
public	BBa_C0061	luxI
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_B0034	1219,1230	BBa_B0034
BBa_C0061	1237,1854	luxI
BBa_B0010	1888,1967	BBa_B0010
BBa_B0012	1976,2016	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I13015/1

Member of these Collections

• //classic/signalling/sender
• iGEM Parts Registry