LOCUS       BBa_I13024              2259 bp    DNA     linear       17-May-2024
DEFINITION  QPI Test Construct Intermediate (Q01121.E0430)
ACCESSION   BBa_I13024
VERSION     BBa_I13024.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     misc_feature    1110..1148
                     /label=LVA
     CDS             21..1148
                     /label=lacI-LVA
     misc_feature    21..1148
                     /label=BBa_C0012
     misc_feature    1149..1173
                     /label=Help:Barcodes
     CDS             21..1148
                     /label=BBa_C0012
     stem_loop       1193..1236
                     /label=stem_loop
     misc_feature    1182..1261
                     /label=BBa_B0010
     terminator      1182..1261
                     /label=BBa_B0010
     stem_loop       1277..1296
                     /label=T7 TE
     misc_feature    1303..1303
                     /label=stop
     polyA_site      1297..1310
                     /label=polya
     misc_feature    1270..1310
                     /label=BBa_B0012
     terminator      1270..1310
                     /label=BBa_B0012
     terminator      1182..1310
                     /label=BBa_B0015
     misc_feature    1321..1337
                     /label=lac O1
     promoter        1338..1343
                     /label=-35
     misc_feature    1344..1360
                     /label=lac O1
     promoter        1361..1366
                     /label=-10
     misc_feature    1319..1372
                     /label=BBa_R0011
     promoter        1319..1372
                     /label=BBa_R0011
     misc_feature    1..1373
                     /label=BBa_Q01121
     misc_feature    1386..1389
                     /label=conserved
     RBS             1382..1393
                     /label=BBa_B0034
     CDS             1400..2122
                     /label=BBa_E0030
     stem_loop       2142..2185
                     /label=stem_loop
     misc_feature    2131..2210
                     /label=BBa_B0010
     terminator      2131..2210
                     /label=BBa_B0010
     stem_loop       2226..2245
                     /label=T7 TE
     misc_feature    2252..2252
                     /label=stop
     polyA_site      2246..2259
                     /label=polya
     misc_feature    2219..2259
                     /label=BBa_B0012
     terminator      2219..2259
                     /label=BBa_B0012
     misc_feature    1382..2259
                     /label=BBa_E0430
ORIGIN
        1 tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
       61 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      121 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      181 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      241 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      301 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      361 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      421 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      481 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      541 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      601 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      661 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      721 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      781 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      841 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      901 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
      961 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1021 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1081 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1141 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1321 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1381 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1441 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1501 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1561 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg
     1621 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1681 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1741 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1801 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1861 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1921 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1981 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     2041 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2101 ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga aaggctcagt
     2161 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2221 acactggctc accttcgggt gggcctttct gcgtttata
//