LOCUS       BBa_I13203              2129 bp    DNA     linear       08-May-2024
DEFINITION  pBad.aiiA protein generator (LVA-)
ACCESSION   BBa_I13203
VERSION     BBa_I13203.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1992
                     /label=aiiA
     CDS             1237..1992
                     /label=BBa_C0160
     stem_loop       2012..2055
                     /label=stem_loop
     misc_feature    2001..2080
                     /label=BBa_B0010
     terminator      2001..2080
                     /label=BBa_B0010
     stem_loop       2096..2115
                     /label=T7 TE
     misc_feature    2122..2122
                     /label=stop
     polyA_site      2116..2129
                     /label=polya
     misc_feature    2089..2129
                     /label=BBa_B0012
     terminator      2089..2129
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga cagtaaagaa gctttatttc
     1261 gtcccagcag gtcgttgtat gttggatcat tcgtctgtta atagtacatt aacaccagga
     1321 gaattattag acttaccggt ttggtgttat cttttggaga ctgaagaagg acctatttta
     1381 gtagatacag gtatgccaga aagtgcagtt aataatgaag gtctttttaa cggtacattt
     1441 gtcgaagggc aggttttacc gaaaatgact gaagaagata gaatcgtgaa tattttaaaa
     1501 cgggttggtt atgagccgga agaccttctt tatattatta gttctcactt gcattttgat
     1561 catgcaggag gaaatggcgc ttttataaat acaccaatca ttgtacagcg tgctgaatat
     1621 gaggcggcgc agcatagcga agaatatttg aaagaatgta tattgccgaa tttaaactac
     1681 aaaatcattg aaggtgatta tgaagtcgta ccaggagttc aattattgca tacaccaggc
     1741 catactccag ggcatcaatc gctattaatt gagacagaaa aatccggtcc tgtattatta
     1801 acgattgatg catcgtatac gaaagagaat tttgaaaatg aagtgccatt tgcgggattt
     1861 gattcagaat tagctttatc ttcaattaaa cgtttaaaag aagtggtgat gaaagagaag
     1921 ccgattgttt tctttggaca tgatatagag caggaaaggg gatgtaaagt gttccctgaa
     1981 tatatataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2041 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     2101 accttcgggt gggcctttct gcgtttata
//