LOCUS       BBa_I13206               973 bp    DNA     linear       03-May-2024
DEFINITION  aiiA (LVA-) protein generator driven by ptet
ACCESSION   BBa_I13206
VERSION     BBa_I13206.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..836
                     /label=aiiA
     CDS             81..836
                     /label=BBa_C0160
     stem_loop       856..899
                     /label=stem_loop
     misc_feature    845..924
                     /label=BBa_B0010
     terminator      845..924
                     /label=BBa_B0010
     stem_loop       940..959
                     /label=T7 TE
     misc_feature    966..966
                     /label=stop
     polyA_site      960..973
                     /label=polya
     misc_feature    933..973
                     /label=BBa_B0012
     terminator      933..973
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgacagtaa agaagcttta tttcgtccca gcaggtcgtt
      121 gtatgttgga tcattcgtct gttaatagta cattaacacc aggagaatta ttagacttac
      181 cggtttggtg ttatcttttg gagactgaag aaggacctat tttagtagat acaggtatgc
      241 cagaaagtgc agttaataat gaaggtcttt ttaacggtac atttgtcgaa gggcaggttt
      301 taccgaaaat gactgaagaa gatagaatcg tgaatatttt aaaacgggtt ggttatgagc
      361 cggaagacct tctttatatt attagttctc acttgcattt tgatcatgca ggaggaaatg
      421 gcgcttttat aaatacacca atcattgtac agcgtgctga atatgaggcg gcgcagcata
      481 gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa ctacaaaatc attgaaggtg
      541 attatgaagt cgtaccagga gttcaattat tgcatacacc aggccatact ccagggcatc
      601 aatcgctatt aattgagaca gaaaaatccg gtcctgtatt attaacgatt gatgcatcgt
      661 atacgaaaga gaattttgaa aatgaagtgc catttgcggg atttgattca gaattagctt
      721 tatcttcaat taaacgttta aaagaagtgg tgatgaaaga gaagccgatt gttttctttg
      781 gacatgatat agagcaggaa aggggatgta aagtgttccc tgaatatata taataatact
      841 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      901 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      961 ttctgcgttt ata
//