LOCUS       BBa_I13208               974 bp    DNA     linear       08-May-2024
DEFINITION  aiiA (LVA-) protein generator driven by plac
ACCESSION   BBa_I13208
VERSION     BBa_I13208.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..837
                     /label=aiiA
     CDS             82..837
                     /label=BBa_C0160
     stem_loop       857..900
                     /label=stem_loop
     misc_feature    846..925
                     /label=BBa_B0010
     terminator      846..925
                     /label=BBa_B0010
     stem_loop       941..960
                     /label=T7 TE
     misc_feature    967..967
                     /label=stop
     polyA_site      961..974
                     /label=polya
     misc_feature    934..974
                     /label=BBa_B0012
     terminator      934..974
                     /label=BBa_B0012
     terminator      846..974
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt
      121 tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt attagactta
      181 ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg
      241 ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt
      301 ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag
      361 ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat
      421 ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat
      481 agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt
      541 gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat
      601 caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg
      661 tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct
      721 ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt
      781 ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat ataataatac
      841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      961 tttctgcgtt tata
//