LOCUS       BBa_I13309              1952 bp    DNA     linear       17-May-2024
DEFINITION  I13303.E0430 Lac-Regulated Rhl Amplifier with YFP Reporter
ACCESSION   BBa_I13309
VERSION     BBa_I13309.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..804
                     /label=rhlR
     misc_feature    805..837
                     /label=LVA
     misc_feature    844..868
                     /label=Help:Barcodes
     CDS             82..843
                     /label=BBa_C0071
     stem_loop       888..931
                     /label=stem_loop
     misc_feature    877..956
                     /label=BBa_B0010
     terminator      877..956
                     /label=BBa_B0010
     stem_loop       972..991
                     /label=T7 TE
     misc_feature    998..998
                     /label=stop
     polyA_site      992..1005
                     /label=polya
     misc_feature    965..1005
                     /label=BBa_B0012
     terminator      965..1005
                     /label=BBa_B0012
     misc_feature    1014..1033
                     /label=RhlR
     promoter        1032..1037
                     /label=-35
     promoter        1055..1060
                     /label=-10
     misc_feature    1066..1066
                     /label=start
     promoter        1014..1066
                     /label=BBa_R0071
     misc_feature    64..1066
                     /label=BBa_I13271
     misc_feature    1..1066
                     /label=BBa_I13303
     misc_feature    1079..1082
                     /label=conserved
     RBS             1075..1086
                     /label=BBa_B0034
     CDS             1093..1815
                     /label=BBa_E0030
     stem_loop       1835..1878
                     /label=stem_loop
     misc_feature    1824..1903
                     /label=BBa_B0010
     terminator      1824..1903
                     /label=BBa_B0010
     stem_loop       1919..1938
                     /label=T7 TE
     misc_feature    1945..1945
                     /label=stop
     polyA_site      1939..1952
                     /label=polya
     misc_feature    1912..1952
                     /label=BBa_B0012
     terminator      1912..1952
                     /label=BBa_B0012
     misc_feature    1075..1952
                     /label=BBa_E0430
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt
      121 ttgcgtagcg agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag
      181 gaagtgcggc gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc
      241 acccggccga agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag
      301 atgcagaact acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg
      361 gtggtctgga gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat
      421 tggggcctct gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg
      481 ctttccgtgg cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg
      541 cggctgcgtt gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg
      601 ctgatgtcca acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc
      661 gacggcaaga gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac
      721 ttccaccaca agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc
      781 tacgccgcgg cgctgggtct catcgctgca aacgacgaaa actacgcttt agtagcttaa
      841 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtcctgtg
     1021 aaatctggca gttaccgtta gctttcgaat tggctaaaaa gtgttctact agagaaagag
     1081 gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1141 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1201 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1261 ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc ccgctacccc
     1321 gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1381 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1441 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1501 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1561 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1621 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1681 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1741 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1801 ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1861 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1921 ctcaccttcg ggtgggcctt tctgcgttta ta
//