LOCUS       BBa_I13312              1991 bp    DNA     linear       10-May-2024
DEFINITION  I13306.E0430 Tet-Regulated Las Amplifier with YFP Reporter
ACCESSION   BBa_I13312
VERSION     BBa_I13312.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=BBa_C0179
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
     misc_feature    994..1011
                     /label=OP2
     misc_feature    1054..1073
                     /label=OP1
     promoter        1065..1070
                     /label=-35
     promoter        1088..1093
                     /label=-10
     promoter        949..1105
                     /label=BBa_R0079
     misc_feature    1118..1121
                     /label=conserved
     RBS             1114..1125
                     /label=BBa_B0034
     CDS             1132..1854
                     /label=BBa_E0030
     stem_loop       1874..1917
                     /label=stem_loop
     misc_feature    1863..1942
                     /label=BBa_B0010
     terminator      1863..1942
                     /label=BBa_B0010
     stem_loop       1958..1977
                     /label=T7 TE
     misc_feature    1984..1984
                     /label=stop
     polyA_site      1978..1991
                     /label=polya
     misc_feature    1951..1991
                     /label=BBa_B0012
     terminator      1951..1991
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa
      121 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga
      181 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg
      241 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc
      301 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc
      361 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc
      421 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag
      481 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc
      541 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac
      601 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca
      661 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa
      721 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt
      781 tgggtcttat tactctctaa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggc ccctcgctga
      961 gcgcgtcccg gagctggggg caacctagct gccacctgct tttctgctag ctattccagc
     1021 gaaaacatac agatttccgg cgaaatcaag gctacctgcc agttctggca ggtttggccg
     1081 cgggttcttt ttggtacacg aaagctacta gagaaagagg agaaatacta gatggtgagc
     1141 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1201 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1261 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1321 accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac
     1381 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1441 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1501 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1561 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1621 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1681 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1741 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1801 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag
     1861 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1921 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1981 ctgcgtttat a
//