LOCUS       BBa_I13313              2124 bp    DNA     linear       17-May-2024
DEFINITION  I13307.E0430 Lac-Regulated Cin Amplifier with YFP Reporter
ACCESSION   BBa_I13313
VERSION     BBa_I13313.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..804
                     /label=cinR
     misc_feature    805..837
                     /label=LVA
     misc_feature    844..868
                     /label=Help:Barcodes
     CDS             82..843
                     /label=BBa_C0077
     stem_loop       888..931
                     /label=stem_loop
     misc_feature    877..956
                     /label=BBa_B0010
     terminator      877..956
                     /label=BBa_B0010
     stem_loop       972..991
                     /label=T7 TE
     misc_feature    998..998
                     /label=stop
     polyA_site      992..1005
                     /label=polya
     misc_feature    965..1005
                     /label=BBa_B0012
     terminator      965..1005
                     /label=BBa_B0012
     misc_feature    1014..1233
                     /label=unidentified/uncharacterized CinR dependent
     stem_loop       1048..1087
                     /label=stem_loop
     stem_loop       1104..1149
                     /label=stem_loop
     promoter        1014..1238
                     /label=BBa_R0078
     misc_feature    64..1238
                     /label=BBa_I13277
     misc_feature    1..1238
                     /label=BBa_I13307
     misc_feature    1251..1254
                     /label=conserved
     RBS             1247..1258
                     /label=BBa_B0034
     CDS             1265..1987
                     /label=BBa_E0030
     stem_loop       2007..2050
                     /label=stem_loop
     misc_feature    1996..2075
                     /label=BBa_B0010
     terminator      1996..2075
                     /label=BBa_B0010
     stem_loop       2091..2110
                     /label=T7 TE
     misc_feature    2117..2117
                     /label=stop
     polyA_site      2111..2124
                     /label=polya
     misc_feature    2084..2124
                     /label=BBa_B0012
     terminator      2084..2124
                     /label=BBa_B0012
     misc_feature    1247..2124
                     /label=BBa_E0430
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgattgag aatacctata gcgaaaagtt cgagtccgcg
      121 ttcgaacaga tcaaggcggc ggccaacgtg gatgccgcca tccgtattct ccaggcggaa
      181 tataacctcg atttcgtcac ctaccatctc gcccagacga tcgcgagcaa gatcgattcg
      241 cccttcgtgc gcaccaccta tccggatgcc tgggtttccc gctacctcct caacagctat
      301 gtgaaggtcg atccgatcgt caagcagggc ttcgaacgcc agctgccctt cgactggagc
      361 gaggtcgaac cgacgccgga ggcctatgcc atgctggtcg acgcccagaa acacggcatc
      421 ggtggcaatg gctactccat ccccgtcgcc gacaaggcgc agcgccgcgc cctgctgtcg
      481 ctgaatgccc gtataccggc cgacgaatgg accgagctcg tgcgccgctg ccgcaacgag
      541 tggatcgaga tcgcccatct gatccaccgc aaggccgtct atgagctgca tggcgaaaac
      601 gatccggtgc cggcattgtc gccgcgcgag atcgagtgtc tgcactggac cgccctcggc
      661 aaggattaca aggatatttc ggtcatcctg ggcatatcag agcataccac acgcgattac
      721 ctgaagaccg cccgcttcaa gctcggctgc gccacgatct cggccgccgc gtcgcgggct
      781 gttcaattgc gcatcatcaa tcccgctgca aacgacgaaa actacgcttt agtagcttaa
      841 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagccctttg
     1021 tgcgtccaaa cggacgcacg gcgctctaaa gcgggtcgcg atctttcaga ttcgctcctc
     1081 gcgctttcag tctttgtttt ggcgcatgtc gttatcgcaa aaccgctgca cacttttgcg
     1141 cgacatgctc tgatccccct catctggggg ggcctatctg agggaatttc cgatccggct
     1201 cgcctgaacc attctgcttt ccacgaactt gaaaacgcta ctagagaaag aggagaaata
     1261 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1321 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1381 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1441 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat
     1501 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1561 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1621 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1681 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1741 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1801 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1861 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1921 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1981 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2041 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2101 cgggtgggcc tttctgcgtt tata
//