LOCUS       BBa_I13402              2961 bp    DNA     linear       17-May-2024
DEFINITION  GFP+Pbad CFP
ACCESSION   BBa_I13402
VERSION     BBa_I13402.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP protein
     CDS             1..720
                     /label=BBa_E0040
     stem_loop       740..783
                     /label=stem_loop
     misc_feature    729..808
                     /label=BBa_B0010
     terminator      729..808
                     /label=BBa_B0010
     stem_loop       824..843
                     /label=T7 TE
     misc_feature    850..850
                     /label=stop
     polyA_site      844..857
                     /label=polya
     misc_feature    817..857
                     /label=BBa_B0012
     terminator      817..857
                     /label=BBa_B0012
     promoter        1945..2075
                     /label=Pbad
     promoter        1908..1979
                     /label=PC
     misc_feature    866..1944
                     /label=AraC
     promoter        866..2075
                     /label=BBa_I0500
     misc_feature    2088..2091
                     /label=conserved
     RBS             2084..2095
                     /label=BBa_B0034
     CDS             2102..2824
                     /label=BBa_E0020
     stem_loop       2844..2887
                     /label=stem_loop
     misc_feature    2833..2912
                     /label=BBa_B0010
     terminator      2833..2912
                     /label=BBa_B0010
     stem_loop       2928..2947
                     /label=T7 TE
     misc_feature    2954..2954
                     /label=stop
     polyA_site      2948..2961
                     /label=polya
     misc_feature    2921..2961
                     /label=BBa_B0012
     terminator      2921..2961
                     /label=BBa_B0012
ORIGIN
        1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      721 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      781 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      841 gcctttctgc gtttatatac tagagttatg acaacttgac ggctacatca ttcacttttt
      901 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      961 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
     1021 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
     1081 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
     1141 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
     1201 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
     1261 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
     1321 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
     1381 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
     1441 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
     1501 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
     1561 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
     1621 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
     1681 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1741 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1801 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1861 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1921 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1981 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     2041 ctactgtttc tccatacccg tttttttggg ctagctacta gagaaagagg agaaatacta
     2101 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2161 cggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2221 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2281 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2341 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2401 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2461 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2521 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
     2581 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2641 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2701 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2761 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2821 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2881 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2941 gtgggccttt ctgcgtttat a
//