LOCUS       BBa_I13405              2982 bp    DNA     linear       04-May-2024
DEFINITION  RBS.YFP + Pbad CFP
ACCESSION   BBa_I13405
VERSION     BBa_I13405.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..741
                     /label=BBa_E0030
     stem_loop       761..804
                     /label=stem_loop
     misc_feature    750..829
                     /label=BBa_B0010
     terminator      750..829
                     /label=BBa_B0010
     stem_loop       845..864
                     /label=T7 TE
     misc_feature    871..871
                     /label=stop
     polyA_site      865..878
                     /label=polya
     misc_feature    838..878
                     /label=BBa_B0012
     terminator      838..878
                     /label=BBa_B0012
     promoter        1966..2096
                     /label=Pbad
     promoter        1929..2000
                     /label=PC
     misc_feature    887..1965
                     /label=AraC
     promoter        887..2096
                     /label=BBa_I0500
     misc_feature    2109..2112
                     /label=conserved
     RBS             2105..2116
                     /label=BBa_B0034
     CDS             2123..2845
                     /label=BBa_E0020
     stem_loop       2865..2908
                     /label=stem_loop
     misc_feature    2854..2933
                     /label=BBa_B0010
     terminator      2854..2933
                     /label=BBa_B0010
     stem_loop       2949..2968
                     /label=T7 TE
     misc_feature    2975..2975
                     /label=stop
     polyA_site      2969..2982
                     /label=polya
     misc_feature    2942..2982
                     /label=BBa_B0012
     terminator      2942..2982
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
      241 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      481 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttat gacaacttga
      901 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
      961 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
     1021 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     1081 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     1141 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     1201 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     1261 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     1321 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     1381 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     1441 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     1501 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     1561 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     1621 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     1681 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     1741 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
     1801 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
     1861 ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga
     1921 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
     1981 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
     2041 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg gctagctact
     2101 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt
     2161 gcccatcctg gtcgagctgg acggcgacgt gaacggccac aagttcagcg tgtccggcga
     2221 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2281 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag
     2341 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2401 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2461 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2521 ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat
     2581 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     2641 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2701 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     2761 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2821 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     2881 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2941 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//