BBa_I13450

BBa_I13450 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I13450
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: jkm
Date created: 2005-05-24 11:00:00
Date modified: 2015-08-31 04:07:34

Removes BioBrick End



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_I13450_sequence (Version 1)

Description

Contains both an early MfeI site and a late NsiI site. Cutting with Mfe and Spe and ligation to a BB part cut EX will form two mixed sites, neither of which can be recut. Cutting with Xba and Nsi and ligation to a BB part cut SP will again form two mixed sites, neither of which can be recut.

Notes

Should be grown in pSB1A2, as Nsi cuts both pSB2K3 and pSB1AK3 and Mfe cuts pSB4A3.
70 bp from Xba to Nsi or Mfe to Spe, the minimum for gel extraction. The middle is largely noise, to raise the size of the part.

Sequence Annotation Location Component / Role(s)
NsiI
MfeI
68,73
2,7
feature/misc sequence_feature
sequence_feature feature/misc
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I13450/1