LOCUS       BBa_I13527               994 bp    DNA     linear       04-May-2024
DEFINITION  Screening plasmid v2 intermediate
ACCESSION   BBa_I13527
VERSION     BBa_I13527.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=RNAse E site
     misc_feature    9..10
                     /label=RNAse E cut site (between the bases)
     misc_feature    22..55
                     /label=Insertion site
     misc_feature    56..76
                     /label=RNAse E site
     misc_feature    64..65
                     /label=RNAse E cut site (between the bases)
     stem_loop       77..111
                     /label=Hairpin
     misc_feature    1..111
                     /label=BBa_I13457
     misc_feature    124..127
                     /label=conserved
     RBS             120..131
                     /label=BBa_B0034
     CDS             138..857
                     /label=GFP protein
     CDS             138..857
                     /label=BBa_E0040
     stem_loop       877..920
                     /label=stem_loop
     misc_feature    866..945
                     /label=BBa_B0010
     terminator      866..945
                     /label=BBa_B0010
     stem_loop       961..980
                     /label=T7 TE
     misc_feature    987..987
                     /label=stop
     polyA_site      981..994
                     /label=polya
     misc_feature    954..994
                     /label=BBa_B0012
     terminator      954..994
                     /label=BBa_B0012
     misc_feature    120..994
                     /label=BBa_I13504
ORIGIN
        1 tattatttgt attgatctgg tgaattaggt cttcgaatcc gatccattgc agtggtatta
       61 tttgtattga tctccttact atctctcgag agattagtac ctttggagat ctactagaga
      121 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
      181 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      241 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      301 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
      361 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      421 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      481 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      541 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      601 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      661 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      721 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      781 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      841 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      901 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      961 ggctcacctt cgggtgggcc tttctgcgtt tata
//