LOCUS       BBa_I13530              2911 bp    DNA     linear       10-May-2024
DEFINITION  Screening Plasmid 2.2
ACCESSION   BBa_I13530
VERSION     BBa_I13530.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1956
                     /label=GFP protein
     CDS             1237..1956
                     /label=BBa_E0040
     misc_feature    1965..2008
                     /label=RNAse E site
     misc_feature    1993..1994
                     /label=RNAse E cut site (between the bases)
     misc_feature    2009..2042
                     /label=Insertion site
     misc_feature    1965..2042
                     /label=BBa_I13455
     misc_feature    2055..2058
                     /label=conserved
     RBS             2051..2062
                     /label=BBa_B0034
     CDS             2069..2743
                     /label=mrfp1
     misc_feature    2750..2774
                     /label=Help:Barcodes
     CDS             2069..2749
                     /label=BBa_E1010
     stem_loop       2794..2837
                     /label=stem_loop
     misc_feature    2783..2862
                     /label=BBa_B0010
     terminator      2783..2862
                     /label=BBa_B0010
     stem_loop       2878..2897
                     /label=T7 TE
     misc_feature    2904..2904
                     /label=stop
     polyA_site      2898..2911
                     /label=polya
     misc_feature    2871..2911
                     /label=BBa_B0012
     terminator      2871..2911
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1921 attacacatg gcatggatga actatacaaa taataatact agagccgcgg tgaaaatacg
     1981 agaatattat ttgtattgat ctcctaggga attaggtctt cgaatccgat ccattgcagt
     2041 ggtactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt
     2101 catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg
     2161 tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg
     2221 tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc
     2281 ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt
     2341 caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc
     2401 ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc
     2461 cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta
     2521 cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg
     2581 tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg
     2641 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt
     2701 tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg
     2761 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     2821 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     2881 tcaccttcgg gtgggccttt ctgcgtttat a
//