LOCUS       BBa_I13531              2888 bp    DNA     linear       13-May-2024
DEFINITION  Screening Plasmid 2.3
ACCESSION   BBa_I13531
VERSION     BBa_I13531.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1911
                     /label=mrfp1
     misc_feature    1918..1942
                     /label=Help:Barcodes
     CDS             1237..1917
                     /label=BBa_E1010
     misc_feature    1951..1971
                     /label=RNAse E site
     misc_feature    1959..1960
                     /label=RNAse E cut site (between the bases)
     misc_feature    1972..2005
                     /label=Insertion site
     misc_feature    1951..2005
                     /label=BBa_I13456
     misc_feature    2018..2021
                     /label=conserved
     RBS             2014..2025
                     /label=BBa_B0034
     CDS             2032..2751
                     /label=GFP protein
     CDS             2032..2751
                     /label=BBa_E0040
     stem_loop       2771..2814
                     /label=stem_loop
     misc_feature    2760..2839
                     /label=BBa_B0010
     terminator      2760..2839
                     /label=BBa_B0010
     stem_loop       2855..2874
                     /label=T7 TE
     misc_feature    2881..2881
                     /label=stop
     polyA_site      2875..2888
                     /label=polya
     misc_feature    2848..2888
                     /label=BBa_B0012
     terminator      2848..2888
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1921 tgatagtgct agtgtagatc gctactagag tattatttgt attgatctgg tgaattaggt
     1981 cttcgaatcc gatccattgc agtggtacta gagaaagagg agaaatacta gatgcgtaaa
     2041 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     2101 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     2161 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     2221 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
     2281 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     2341 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     2401 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     2461 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     2521 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     2581 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     2641 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     2701 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
     2761 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2821 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2881 cgtttata
//