LOCUS       BBa_I13532              2888 bp    DNA     linear       10-May-2024
DEFINITION  Screening Plasmid 2.4
ACCESSION   BBa_I13532
VERSION     BBa_I13532.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1956
                     /label=GFP protein
     CDS             1237..1956
                     /label=BBa_E0040
     misc_feature    1965..1985
                     /label=RNAse E site
     misc_feature    1973..1974
                     /label=RNAse E cut site (between the bases)
     misc_feature    1986..2019
                     /label=Insertion site
     misc_feature    1965..2019
                     /label=BBa_I13456
     misc_feature    2032..2035
                     /label=conserved
     RBS             2028..2039
                     /label=BBa_B0034
     CDS             2046..2720
                     /label=mrfp1
     misc_feature    2727..2751
                     /label=Help:Barcodes
     CDS             2046..2726
                     /label=BBa_E1010
     stem_loop       2771..2814
                     /label=stem_loop
     misc_feature    2760..2839
                     /label=BBa_B0010
     terminator      2760..2839
                     /label=BBa_B0010
     stem_loop       2855..2874
                     /label=T7 TE
     misc_feature    2881..2881
                     /label=stop
     polyA_site      2875..2888
                     /label=polya
     misc_feature    2848..2888
                     /label=BBa_B0012
     terminator      2848..2888
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1921 attacacatg gcatggatga actatacaaa taataatact agagtattat ttgtattgat
     1981 ctggtgaatt aggtcttcga atccgatcca ttgcagtggt actagagaaa gaggagaaat
     2041 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2101 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2161 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     2221 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     2281 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     2341 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     2401 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     2461 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     2521 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     2581 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     2641 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     2701 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     2761 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2821 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2881 cgtttata
//