LOCUS       BBa_I13537              3261 bp    DNA     linear       12-May-2024
DEFINITION  Two promoter screening plasmid v1.0
ACCESSION   BBa_I13537
VERSION     BBa_I13537.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1114
                     /label=BBa_I13458
     promoter        1123..1252
                     /label=BBa_I13453
     misc_feature    1261..1281
                     /label=RNAse E site
     misc_feature    1269..1270
                     /label=RNAse E cut site (between the bases)
     misc_feature    1282..1315
                     /label=Insertion site
     misc_feature    1316..1336
                     /label=RNAse E site
     misc_feature    1324..1325
                     /label=RNAse E cut site (between the bases)
     stem_loop       1337..1371
                     /label=Hairpin
     misc_feature    1261..1371
                     /label=BBa_I13457
     misc_feature    1384..1387
                     /label=conserved
     RBS             1380..1391
                     /label=BBa_B0034
     CDS             1398..2072
                     /label=mrfp1
     misc_feature    2079..2103
                     /label=Help:Barcodes
     CDS             1398..2078
                     /label=BBa_E1010
     stem_loop       2123..2166
                     /label=stem_loop
     misc_feature    2112..2191
                     /label=BBa_B0010
     terminator      2112..2191
                     /label=BBa_B0010
     stem_loop       2207..2226
                     /label=T7 TE
     misc_feature    2233..2233
                     /label=stop
     polyA_site      2227..2240
                     /label=polya
     misc_feature    2200..2240
                     /label=BBa_B0012
     terminator      2200..2240
                     /label=BBa_B0012
     promoter        2249..2378
                     /label=BBa_I13453
     misc_feature    2391..2394
                     /label=conserved
     RBS             2387..2398
                     /label=BBa_B0034
     CDS             2405..3124
                     /label=GFP protein
     CDS             2405..3124
                     /label=BBa_E0040
     stem_loop       3144..3187
                     /label=stem_loop
     misc_feature    3133..3212
                     /label=BBa_B0010
     terminator      3133..3212
                     /label=BBa_B0010
     stem_loop       3228..3247
                     /label=T7 TE
     misc_feature    3254..3254
                     /label=stop
     polyA_site      3248..3261
                     /label=polya
     misc_feature    3221..3261
                     /label=BBa_B0012
     terminator      3221..3261
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg
     1141 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag
     1261 tattatttgt attgatctgg tgaattaggt cttcgaatcc gatccattgc agtggtatta
     1321 tttgtattga tctccttact atctctcgag agattagtac ctttggagat ctactagaga
     1381 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca
     1441 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag
     1501 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc
     1561 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac
     1621 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac
     1681 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag
     1741 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg
     1801 ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg
     1861 gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg
     1921 ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa
     1981 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg
     2041 aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat
     2101 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2161 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2221 tgggcctttc tgcgtttata tactagagac attgattatt tgcacggcgt cacactttgc
     2281 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
     2341 ctctctactg tttctccata ccgttttttt gggctagcta ctagagaaag aggagaaata
     2401 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     2461 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     2521 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     2581 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     2641 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     2701 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     2761 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     2821 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     2881 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     2941 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     3001 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     3061 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     3121 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     3181 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     3241 gtgggccttt ctgcgtttat a
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