LOCUS       BBa_I13541              1013 bp    DNA     linear       04-May-2024
DEFINITION  Intermediate in two promoter screening plasmid
ACCESSION   BBa_I13541
VERSION     BBa_I13541.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..876
                     /label=GFP protein
     CDS             157..876
                     /label=BBa_E0040
     stem_loop       896..939
                     /label=stem_loop
     misc_feature    885..964
                     /label=BBa_B0010
     terminator      885..964
                     /label=BBa_B0010
     stem_loop       980..999
                     /label=T7 TE
     misc_feature    1006..1006
                     /label=stop
     polyA_site      1000..1013
                     /label=polya
     misc_feature    973..1013
                     /label=BBa_B0012
     terminator      973..1013
                     /label=BBa_B0012
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
      181 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      241 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      301 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
      361 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
      421 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      481 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      541 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
      601 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
      661 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      721 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
      781 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
      841 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
      901 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      961 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//