LOCUS       BBa_I13542              1118 bp    DNA     linear       03-May-2024
DEFINITION  Intermediate in two promoter screening plasmid
ACCESSION   BBa_I13542
VERSION     BBa_I13542.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    139..159
                     /label=RNAse E site
     misc_feature    147..148
                     /label=RNAse E cut site (between the bases)
     misc_feature    160..193
                     /label=Insertion site
     misc_feature    194..214
                     /label=RNAse E site
     misc_feature    202..203
                     /label=RNAse E cut site (between the bases)
     stem_loop       215..249
                     /label=Hairpin
     misc_feature    139..249
                     /label=BBa_I13457
     misc_feature    262..265
                     /label=conserved
     RBS             258..269
                     /label=BBa_B0034
     CDS             276..950
                     /label=mrfp1
     misc_feature    957..981
                     /label=Help:Barcodes
     CDS             276..956
                     /label=BBa_E1010
     stem_loop       1001..1044
                     /label=stem_loop
     misc_feature    990..1069
                     /label=BBa_B0010
     terminator      990..1069
                     /label=BBa_B0010
     stem_loop       1085..1104
                     /label=T7 TE
     misc_feature    1111..1111
                     /label=stop
     polyA_site      1105..1118
                     /label=polya
     misc_feature    1078..1118
                     /label=BBa_B0012
     terminator      1078..1118
                     /label=BBa_B0012
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagta ttatttgtat tgatctggtg aattaggtct tcgaatccga
      181 tccattgcag tggtattatt tgtattgatc tccttactat ctctcgagag attagtacct
      241 ttggagatct actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
      301 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
      361 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
      421 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
      481 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
      541 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
      601 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
      661 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
      721 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
      781 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
      841 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
      901 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
      961 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1081 cactggctca ccttcgggtg ggcctttctg cgtttata
//