LOCUS       BBa_I13573               966 bp    DNA     linear       17-May-2024
DEFINITION  Ptet + RFP with 5' hairpin + Terminator
ACCESSION   BBa_I13573
VERSION     BBa_I13573.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     stem_loop       63..97
                     /label=Hairpin
     misc_feature    63..97
                     /label=BBa_I13577
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..798
                     /label=mrfp1
     misc_feature    805..829
                     /label=Help:Barcodes
     CDS             124..829
                     /label=BBa_E1010
     stem_loop       849..892
                     /label=stem_loop
     misc_feature    838..917
                     /label=BBa_B0010
     terminator      838..917
                     /label=BBa_B0010
     stem_loop       933..952
                     /label=T7 TE
     misc_feature    959..959
                     /label=stop
     polyA_site      953..966
                     /label=polya
     misc_feature    926..966
                     /label=BBa_B0012
     terminator      926..966
                     /label=BBa_B0012
     terminator      838..966
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtactatct ctcgagagat tagtaccttt ggagatctac tagagaaaga ggagaaatac
      121 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      181 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      241 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      301 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      361 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      421 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      481 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      541 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      601 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      661 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      721 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      781 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttata
//