LOCUS       BBa_I13611              2282 bp    DNA     linear       17-May-2024
DEFINITION  Cascaded Tet (weak) and Lac (medium) QPIs
ACCESSION   BBa_I13611
VERSION     BBa_I13611.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     CDS             21..637
                     /label=tetR
     misc_feature    638..671
                     /label=SsrA
     misc_feature    678..702
                     /label=Help:Barcodes
     CDS             18..677
                     /label=BBa_C0040
     stem_loop       722..765
                     /label=stem_loop
     misc_feature    711..790
                     /label=BBa_B0010
     terminator      711..790
                     /label=BBa_B0010
     stem_loop       806..825
                     /label=T7 TE
     misc_feature    832..832
                     /label=stop
     polyA_site      826..839
                     /label=polya
     misc_feature    799..839
                     /label=BBa_B0012
     terminator      799..839
                     /label=BBa_B0012
     misc_feature    848..866
                     /label=TetR 1
     misc_feature    848..901
                     /label=BBa_R0040
     promoter        867..872
                     /label=-35
     misc_feature    873..891
                     /label=TetR 2
     promoter        890..895
                     /label=-10
     promoter        848..901
                     /label=BBa_R0040
     misc_feature    1..901
                     /label=BBa_Q03400
     misc_feature    916..919
                     /label=conserved
     RBS             910..923
                     /label=BBa_B0031
     misc_feature    2019..2057
                     /label=LVA
     CDS             930..2057
                     /label=lacI-LVA
     misc_feature    930..2057
                     /label=BBa_C0012
     misc_feature    2058..2082
                     /label=Help:Barcodes
     CDS             930..2057
                     /label=BBa_C0012
     stem_loop       2102..2145
                     /label=stem_loop
     misc_feature    2091..2170
                     /label=BBa_B0010
     terminator      2091..2170
                     /label=BBa_B0010
     stem_loop       2186..2205
                     /label=T7 TE
     misc_feature    2212..2212
                     /label=stop
     polyA_site      2206..2219
                     /label=polya
     misc_feature    2179..2219
                     /label=BBa_B0012
     terminator      2179..2219
                     /label=BBa_B0012
     terminator      2091..2219
                     /label=BBa_B0015
     misc_feature    2230..2246
                     /label=lac O1
     promoter        2247..2252
                     /label=-35
     misc_feature    2253..2269
                     /label=lac O1
     promoter        2270..2275
                     /label=-10
     misc_feature    2228..2281
                     /label=BBa_R0011
     promoter        2228..2281
                     /label=BBa_R0011
     misc_feature    910..2282
                     /label=BBa_Q01121
ORIGIN
        1 tcacacagga ctactagatg tccagattag ataaaagtaa agtgattaac agcgcattag
       61 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
      121 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
      181 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
      241 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
      301 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
      361 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
      421 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
      481 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
      541 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
      601 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg
      661 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca
      721 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      781 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      841 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
      901 ctactagagt cacacaggaa acctactaga tggtgaatgt gaaaccagta acgttatacg
      961 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     1021 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     1081 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     1141 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     1201 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     1261 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     1321 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     1381 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     1441 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     1501 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     1561 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     1621 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     1681 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     1741 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     1801 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     1861 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     1921 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1981 agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg
     2041 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ccaggcatca
     2101 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2161 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     2221 actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag atactgagca
     2281 ca
//