LOCUS       BBa_I13614              2285 bp    DNA     linear       17-May-2024
DEFINITION  Cascaded Tet (medium) and Lac (medium) QPIs
ACCESSION   BBa_I13614
VERSION     BBa_I13614.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             24..640
                     /label=tetR
     misc_feature    641..674
                     /label=SsrA
     misc_feature    681..705
                     /label=Help:Barcodes
     CDS             21..680
                     /label=BBa_C0040
     stem_loop       725..768
                     /label=stem_loop
     misc_feature    714..793
                     /label=BBa_B0010
     terminator      714..793
                     /label=BBa_B0010
     stem_loop       809..828
                     /label=T7 TE
     misc_feature    835..835
                     /label=stop
     polyA_site      829..842
                     /label=polya
     misc_feature    802..842
                     /label=BBa_B0012
     terminator      802..842
                     /label=BBa_B0012
     misc_feature    851..869
                     /label=TetR 1
     misc_feature    851..904
                     /label=BBa_R0040
     promoter        870..875
                     /label=-35
     misc_feature    876..894
                     /label=TetR 2
     promoter        893..898
                     /label=-10
     promoter        851..904
                     /label=BBa_R0040
     misc_feature    1..904
                     /label=BBa_Q01400
     misc_feature    919..922
                     /label=conserved
     RBS             913..926
                     /label=BBa_B0031
     misc_feature    2022..2060
                     /label=LVA
     CDS             933..2060
                     /label=lacI-LVA
     misc_feature    933..2060
                     /label=BBa_C0012
     misc_feature    2061..2085
                     /label=Help:Barcodes
     CDS             933..2060
                     /label=BBa_C0012
     stem_loop       2105..2148
                     /label=stem_loop
     misc_feature    2094..2173
                     /label=BBa_B0010
     terminator      2094..2173
                     /label=BBa_B0010
     stem_loop       2189..2208
                     /label=T7 TE
     misc_feature    2215..2215
                     /label=stop
     polyA_site      2209..2222
                     /label=polya
     misc_feature    2182..2222
                     /label=BBa_B0012
     terminator      2182..2222
                     /label=BBa_B0012
     terminator      2094..2222
                     /label=BBa_B0015
     misc_feature    2233..2249
                     /label=lac O1
     promoter        2250..2255
                     /label=-35
     misc_feature    2256..2272
                     /label=lac O1
     promoter        2273..2278
                     /label=-10
     misc_feature    2231..2284
                     /label=BBa_R0011
     promoter        2231..2284
                     /label=BBa_R0011
     misc_feature    913..2285
                     /label=BBa_Q01121
ORIGIN
        1 tcacacagga aacctactag atgtccagat tagataaaag taaagtgatt aacagcgcat
       61 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      121 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      181 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      241 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      301 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      361 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      421 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      481 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      541 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      601 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      661 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca
      721 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      781 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      841 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      901 gcactactag agtcacacag gaaacctact agatggtgaa tgtgaaacca gtaacgttat
      961 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
     1021 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
     1081 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
     1141 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
     1201 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
     1261 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
     1321 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
     1381 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
     1441 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
     1501 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
     1561 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
     1621 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg
     1681 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
     1741 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
     1801 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     1861 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     1921 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1981 tgcagctggc acgacaggtt tcccgactgg aaagcgggca ggctgcaaac gacgaaaact
     2041 acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca
     2101 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2161 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2221 tatactagag aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga
     2281 gcaca
//