LOCUS       BBa_I13617              2283 bp    DNA     linear       17-May-2024
DEFINITION  Cascaded Tet (elowitz) and Lac (medium) QPIs
ACCESSION   BBa_I13617
VERSION     BBa_I13617.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     stem_loop       723..766
                     /label=stem_loop
     misc_feature    712..791
                     /label=BBa_B0010
     terminator      712..791
                     /label=BBa_B0010
     stem_loop       807..826
                     /label=T7 TE
     misc_feature    833..833
                     /label=stop
     polyA_site      827..840
                     /label=polya
     misc_feature    800..840
                     /label=BBa_B0012
     terminator      800..840
                     /label=BBa_B0012
     misc_feature    849..867
                     /label=TetR 1
     misc_feature    849..902
                     /label=BBa_R0040
     promoter        868..873
                     /label=-35
     misc_feature    874..892
                     /label=TetR 2
     promoter        891..896
                     /label=-10
     promoter        849..902
                     /label=BBa_R0040
     misc_feature    1..902
                     /label=BBa_Q04400
     misc_feature    917..920
                     /label=conserved
     RBS             911..924
                     /label=BBa_B0031
     misc_feature    2020..2058
                     /label=LVA
     CDS             931..2058
                     /label=lacI-LVA
     misc_feature    931..2058
                     /label=BBa_C0012
     misc_feature    2059..2083
                     /label=Help:Barcodes
     CDS             931..2058
                     /label=BBa_C0012
     stem_loop       2103..2146
                     /label=stem_loop
     misc_feature    2092..2171
                     /label=BBa_B0010
     terminator      2092..2171
                     /label=BBa_B0010
     stem_loop       2187..2206
                     /label=T7 TE
     misc_feature    2213..2213
                     /label=stop
     polyA_site      2207..2220
                     /label=polya
     misc_feature    2180..2220
                     /label=BBa_B0012
     terminator      2180..2220
                     /label=BBa_B0012
     terminator      2092..2220
                     /label=BBa_B0015
     misc_feature    2231..2247
                     /label=lac O1
     promoter        2248..2253
                     /label=-35
     misc_feature    2254..2270
                     /label=lac O1
     promoter        2271..2276
                     /label=-10
     misc_feature    2229..2282
                     /label=BBa_R0011
     promoter        2229..2282
                     /label=BBa_R0011
     misc_feature    911..2283
                     /label=BBa_Q01121
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
      721 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      781 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
      841 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      901 actactagag tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac
      961 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
     1021 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
     1081 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
     1141 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
     1201 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
     1261 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
     1321 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
     1381 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
     1441 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
     1501 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
     1561 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
     1621 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
     1681 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
     1741 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
     1801 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     1861 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     1921 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1981 cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac
     2041 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc
     2101 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2161 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2221 tactagagaa ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc
     2281 aca
//