LOCUS       BBa_I13621              2282 bp    DNA     linear       17-May-2024
DEFINITION  Cascaded Lac (weak) and Tet (medium) QPIs
ACCESSION   BBa_I13621
VERSION     BBa_I13621.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     misc_feature    1107..1145
                     /label=LVA
     CDS             18..1145
                     /label=lacI-LVA
     misc_feature    18..1145
                     /label=BBa_C0012
     misc_feature    1146..1170
                     /label=Help:Barcodes
     CDS             18..1145
                     /label=BBa_C0012
     stem_loop       1190..1233
                     /label=stem_loop
     misc_feature    1179..1258
                     /label=BBa_B0010
     terminator      1179..1258
                     /label=BBa_B0010
     stem_loop       1274..1293
                     /label=T7 TE
     misc_feature    1300..1300
                     /label=stop
     polyA_site      1294..1307
                     /label=polya
     misc_feature    1267..1307
                     /label=BBa_B0012
     terminator      1267..1307
                     /label=BBa_B0012
     terminator      1179..1307
                     /label=BBa_B0015
     misc_feature    1318..1334
                     /label=lac O1
     promoter        1335..1340
                     /label=-35
     misc_feature    1341..1357
                     /label=lac O1
     promoter        1358..1363
                     /label=-10
     misc_feature    1316..1369
                     /label=BBa_R0011
     promoter        1316..1369
                     /label=BBa_R0011
     misc_feature    1..1370
                     /label=BBa_Q03121
     misc_feature    1385..1388
                     /label=conserved
     RBS             1379..1392
                     /label=BBa_B0031
     CDS             1402..2018
                     /label=tetR
     misc_feature    2019..2052
                     /label=SsrA
     misc_feature    2059..2083
                     /label=Help:Barcodes
     CDS             1399..2058
                     /label=BBa_C0040
     stem_loop       2103..2146
                     /label=stem_loop
     misc_feature    2092..2171
                     /label=BBa_B0010
     terminator      2092..2171
                     /label=BBa_B0010
     stem_loop       2187..2206
                     /label=T7 TE
     misc_feature    2213..2213
                     /label=stop
     polyA_site      2207..2220
                     /label=polya
     misc_feature    2180..2220
                     /label=BBa_B0012
     terminator      2180..2220
                     /label=BBa_B0012
     misc_feature    2229..2247
                     /label=TetR 1
     misc_feature    2229..2282
                     /label=BBa_R0040
     promoter        2248..2253
                     /label=-35
     misc_feature    2254..2272
                     /label=TetR 2
     promoter        2271..2276
                     /label=-10
     promoter        2229..2282
                     /label=BBa_R0040
     misc_feature    1379..2282
                     /label=BBa_Q01400
ORIGIN
        1 tcacacagga ctactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
       61 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
      121 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
      181 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
      241 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
      301 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
      361 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
      421 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
      481 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
      541 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
      601 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
      661 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
      721 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
      781 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
      841 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
      901 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
      961 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     1021 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1081 aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt
     1141 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
     1201 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1261 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaattg
     1321 tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca tactagagtc
     1381 acacaggaaa cctactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
     1441 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
     1501 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
     1561 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
     1621 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
     1681 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
     1741 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
     1801 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
     1861 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
     1921 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
     1981 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
     2041 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
     2101 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2161 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2221 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     2281 ac
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