LOCUS       BBa_I13730              2410 bp    DNA     linear       14-Jun-2024
DEFINITION  Lac CheB, Tet CheR
ACCESSION   BBa_I13730
VERSION     BBa_I13730.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1134
                     /label=CheB
     misc_feature    1135..1159
                     /label=Help:Barcodes
     CDS             82..1159
                     /label=BBa_C0024
     stem_loop       1179..1222
                     /label=stem_loop
     misc_feature    1168..1247
                     /label=BBa_B0010
     terminator      1168..1247
                     /label=BBa_B0010
     stem_loop       1263..1282
                     /label=T7 TE
     misc_feature    1289..1289
                     /label=stop
     polyA_site      1283..1296
                     /label=polya
     misc_feature    1256..1296
                     /label=BBa_B0012
     terminator      1256..1296
                     /label=BBa_B0012
     terminator      1168..1296
                     /label=BBa_B0015
     misc_feature    1..1296
                     /label=BBa_I13721
     misc_feature    1305..1323
                     /label=TetR 1
     misc_feature    1305..1358
                     /label=BBa_R0040
     promoter        1324..1329
                     /label=-35
     misc_feature    1330..1348
                     /label=TetR 2
     promoter        1347..1352
                     /label=-10
     promoter        1305..1358
                     /label=BBa_R0040
     misc_feature    1371..1374
                     /label=conserved
     RBS             1367..1378
                     /label=BBa_B0034
     CDS             1385..2248
                     /label=CheB
     misc_feature    2249..2273
                     /label=Help:Barcodes
     CDS             1385..2248
                     /label=BBa_C0028
     stem_loop       2293..2336
                     /label=stem_loop
     misc_feature    2282..2361
                     /label=BBa_B0010
     terminator      2282..2361
                     /label=BBa_B0010
     stem_loop       2377..2396
                     /label=T7 TE
     misc_feature    2403..2403
                     /label=stop
     polyA_site      2397..2410
                     /label=polya
     misc_feature    2370..2410
                     /label=BBa_B0012
     terminator      2370..2410
                     /label=BBa_B0012
     misc_feature    1305..2410
                     /label=BBa_I13712
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcaaa atcagggtgt tatctgtcga tgattcggca
      121 ctgatgcgcc agatcatgac agaaatcatc aacagccata gcgacatgga aatggtggcg
      181 accgcgcctg atccgctggt cgcgcgtgac ttgattaaga aattcaatcc cgatgtgctg
      241 acgctggatg ttgaaatgcc gcggatggac ggactggatt tcctcgaaaa attaatgcgt
      301 ttgcgtccaa tgcccgttgt gatggtttct tccctgaccg gcaaagggtc agaagtcacg
      361 ctgcgcgcgc tggagctggg ggcgatagat tttgtcacca aaccgcaact gggtattcgc
      421 gaaggtatgc tggcgtataa cgaaatgatt gctgaaaagg tgcgtacggc agcaaaggcg
      481 agccttgcag cacataagcc attgtcggca ccgacaacgc tgaaggcggg gccgttgttg
      541 agttctgaaa aactgattgc gattggtgct tcaacgggtg gaactgaggc aattcgtcac
      601 gtactgcaac cgttgccgct ttccagcccg gcactgttaa ttacccagca tatgccgccc
      661 ggtttcaccc gctcttttgc cgacagactt aataagcttt gccagatcgg ggttaaagaa
      721 gccgaagacg gagaacgtgt cttgccgggg catgcctata ttgcgccggg cgatcggcat
      781 atggagctgt cgcgtagtgg cgcaaattac caaatcaaaa ttcacgatgg cccggcggtt
      841 aaccgtcatc ggccttcggt agatgtgttg ttccattctg tcgccaaaca ggcggggcgt
      901 aatgcggttg gggtgatcct gaccggtatg ggcaacgacg gcgcggcggg aatgttggcg
      961 atgcgtcagg cgggggcatg gacccttgcg caaaacgaag caagttgcgt ggtgttcggc
     1021 atgccgcgcg aggccatcaa tatgggtggt gtctgcgaag tggtcgatct tagccaggta
     1081 agccagcaaa tgttggcaaa aattagtgcc ggacaggcga tacgtattta ataacactga
     1141 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1201 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1261 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag
     1321 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata
     1381 ctagatgact tcatctctgc cctgtgggca aacgtcttta ttgttacaga tgaccgagcg
     1441 cctggcgctt tccgacgcgc attttcggcg gataagtcaa ttgatctatc aacgagccgg
     1501 gatcgttctg gctgaccata aacgcgacat ggtttacaac cgactggttc gtcgtttgcg
     1561 ttcgctggga ctgacggatt tcggtcatta tctgaacttg ctggaatcta atcagcacag
     1621 cggtgagtgg caggcgttta tcaattcgct gaccacgaat ctgacggcat ttttccgtga
     1681 ggcacatcat ttccctctgc tcgcggatca cgcacgtcgc cgttctggcg agtatcgcgt
     1741 atggagcgcg gcggcttcga ccggcgaaga gccgtacagc attgcgatga cgctggctga
     1801 cacattgggc accgcgcccg gacgctggaa agtgtttgcc agtgatatcg acaccgaagt
     1861 gctggaaaaa gccagaagcg gtatctatcg ccatgaagag ttgaaaaacc tgacgccgca
     1921 gcaactgcaa cggtatttca tgcgagggac ggggccgcat gaagggctgg tacgcgtgcg
     1981 tcaggagctg gcgaactatg ttgattttgc cccgctgaat ctactggcga aacagtacac
     2041 cgtgccgggg ccgtttgatg cgatcttctg tcgtaacgtc atgatctact tcgatcaaac
     2101 tacccagcag gagattttgc gccgctttgt tccgctcctt aaacccgacg gattgctgtt
     2161 tgcgggtcac tctgaaaact ttagccacct tgagcgccgc ttcacgctgc gtggtcagac
     2221 ggtgtatgcg ctaagtaagg attaataacg ctgatagtgc tagtgtagat cgctactaga
     2281 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2341 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2401 tgcgtttata
//