BBa_I13721 1 BBa_I13721 CheB under Lac control with Elowitz RBS 2004-08-12T11:00:00Z 2015-08-31T04:07:36Z -- No description -- false false _6_ 0 101 7 Not in stock false false Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante component2221651 1 BBa_B0015 component2221644 1 BBa_C0024 component2221641 1 BBa_B0034 component2221635 1 BBa_R0011 annotation2221635 1 BBa_R0011 range2221635 1 1 54 annotation2221641 1 BBa_B0034 range2221641 1 64 75 annotation2221651 1 BBa_B0015 range2221651 1 1168 1296 annotation2221644 1 BBa_C0024 range2221644 1 82 1159 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_C0028 1 cher CheR chemotaxis coding region 2004-08-01T11:00:00Z 2015-08-31T04:07:23Z NCBI - E. Coli K12 substrain: MG1655 CheR is part of the chemotaxis pathway in E. Coli. (Was part # I13702) false false _6_ 0 101 7 Not in stock false Checked (DE, VC, KN). Part ready for synthesis (8/3/4) <P>sequence data at http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946396 false MIT SBC 2004 (Ken) annotation2214018 1 Help:Barcodes range2214018 1 865 889 annotation1891584 1 CheB range1891584 1 1 864 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2000 1 -35 range2000 1 20 25 annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2002 1 -10 range2002 1 43 48 annotation1999 1 lac O1 range1999 1 3 19 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I13730 1 BBa_I13730 Lac CheB, Tet CheR 2004-08-12T11:00:00Z 2015-08-31T04:07:36Z -- No description -- false false _6_ 0 101 7 Not in stock false false Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante component2229672 1 BBa_I13721 component2229688 1 BBa_I13712 annotation2229672 1 BBa_I13721 range2229672 1 1 1296 annotation2229688 1 BBa_I13712 range2229688 1 1305 2410 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986787 1 -10 range1986787 1 43 48 annotation1986785 1 -35 range1986785 1 20 25 BBa_C0024 1 cheB CheB chemotaxis coding sequence (protein glutamate methylesterase) 2004-08-01T11:00:00Z 2015-08-31T04:07:23Z NCBI - E. Coli strain K12 substrain MG1655 Released HQ 2013 CheB is part of the chemotaxis pathway in E. coli. false false _6_ 0 101 7 In stock false Checked (DE, VC, KN). Part ready for synthesis. 8/3/4 true MIT SBC 2004 (Ken) annotation1891614 1 CheB range1891614 1 1 1053 annotation2214017 1 Help:Barcodes range2214017 1 1054 1078 BBa_I13712 1 BBa_I13712 CheR behind a tet promoter and Elowitz RBS 2004-08-12T11:00:00Z 2015-08-31T04:07:36Z -- No description -- false false _6_ 0 101 7 Not in stock false false Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante component1045364 1 BBa_B0034 component1045356 1 BBa_R0040 component1045368 1 BBa_C0028 component1045384 1 BBa_B0012 component1045374 1 BBa_B0010 annotation1045368 1 BBa_C0028 range1045368 1 81 944 annotation1045364 1 BBa_B0034 range1045364 1 63 74 annotation1045384 1 BBa_B0012 range1045384 1 1066 1106 annotation1045356 1 BBa_R0040 range1045356 1 1 54 annotation1045374 1 BBa_B0010 range1045374 1 978 1057 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_C0028_sequence 1 atgacttcatctctgccctgtgggcaaacgtctttattgttacagatgaccgagcgcctggcgctttccgacgcgcattttcggcggataagtcaattgatctatcaacgagccgggatcgttctggctgaccataaacgcgacatggtttacaaccgactggttcgtcgtttgcgttcgctgggactgacggatttcggtcattatctgaacttgctggaatctaatcagcacagcggtgagtggcaggcgtttatcaattcgctgaccacgaatctgacggcatttttccgtgaggcacatcatttccctctgctcgcggatcacgcacgtcgccgttctggcgagtatcgcgtatggagcgcggcggcttcgaccggcgaagagccgtacagcattgcgatgacgctggctgacacattgggcaccgcgcccggacgctggaaagtgtttgccagtgatatcgacaccgaagtgctggaaaaagccagaagcggtatctatcgccatgaagagttgaaaaacctgacgccgcagcaactgcaacggtatttcatgcgagggacggggccgcatgaagggctggtacgcgtgcgtcaggagctggcgaactatgttgattttgccccgctgaatctactggcgaaacagtacaccgtgccggggccgtttgatgcgatcttctgtcgtaacgtcatgatctacttcgatcaaactacccagcaggagattttgcgccgctttgttccgctccttaaacccgacggattgctgtttgcgggtcactctgaaaactttagccaccttgagcgccgcttcacgctgcgtggtcagacggtgtatgcgctaagtaaggattaataacgctgatagtgctagtgtagatcgc BBa_C0024_sequence 1 atgagcaaaatcagggtgttatctgtcgatgattcggcactgatgcgccagatcatgacagaaatcatcaacagccatagcgacatggaaatggtggcgaccgcgcctgatccgctggtcgcgcgtgacttgattaagaaattcaatcccgatgtgctgacgctggatgttgaaatgccgcggatggacggactggatttcctcgaaaaattaatgcgtttgcgtccaatgcccgttgtgatggtttcttccctgaccggcaaagggtcagaagtcacgctgcgcgcgctggagctgggggcgatagattttgtcaccaaaccgcaactgggtattcgcgaaggtatgctggcgtataacgaaatgattgctgaaaaggtgcgtacggcagcaaaggcgagccttgcagcacataagccattgtcggcaccgacaacgctgaaggcggggccgttgttgagttctgaaaaactgattgcgattggtgcttcaacgggtggaactgaggcaattcgtcacgtactgcaaccgttgccgctttccagcccggcactgttaattacccagcatatgccgcccggtttcacccgctcttttgccgacagacttaataagctttgccagatcggggttaaagaagccgaagacggagaacgtgtcttgccggggcatgcctatattgcgccgggcgatcggcatatggagctgtcgcgtagtggcgcaaattaccaaatcaaaattcacgatggcccggcggttaaccgtcatcggccttcggtagatgtgttgttccattctgtcgccaaacaggcggggcgtaatgcggttggggtgatcctgaccggtatgggcaacgacggcgcggcgggaatgttggcgatgcgtcaggcgggggcatggacccttgcgcaaaacgaagcaagttgcgtggtgttcggcatgccgcgcgaggccatcaatatgggtggtgtctgcgaagtggtcgatcttagccaggtaagccagcaaatgttggcaaaaattagtgccggacaggcgatacgtatttaataacactgatagtgctagtgtagatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_I13721_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcaaaatcagggtgttatctgtcgatgattcggcactgatgcgccagatcatgacagaaatcatcaacagccatagcgacatggaaatggtggcgaccgcgcctgatccgctggtcgcgcgtgacttgattaagaaattcaatcccgatgtgctgacgctggatgttgaaatgccgcggatggacggactggatttcctcgaaaaattaatgcgtttgcgtccaatgcccgttgtgatggtttcttccctgaccggcaaagggtcagaagtcacgctgcgcgcgctggagctgggggcgatagattttgtcaccaaaccgcaactgggtattcgcgaaggtatgctggcgtataacgaaatgattgctgaaaaggtgcgtacggcagcaaaggcgagccttgcagcacataagccattgtcggcaccgacaacgctgaaggcggggccgttgttgagttctgaaaaactgattgcgattggtgcttcaacgggtggaactgaggcaattcgtcacgtactgcaaccgttgccgctttccagcccggcactgttaattacccagcatatgccgcccggtttcacccgctcttttgccgacagacttaataagctttgccagatcggggttaaagaagccgaagacggagaacgtgtcttgccggggcatgcctatattgcgccgggcgatcggcatatggagctgtcgcgtagtggcgcaaattaccaaatcaaaattcacgatggcccggcggttaaccgtcatcggccttcggtagatgtgttgttccattctgtcgccaaacaggcggggcgtaatgcggttggggtgatcctgaccggtatgggcaacgacggcgcggcgggaatgttggcgatgcgtcaggcgggggcatggacccttgcgcaaaacgaagcaagttgcgtggtgttcggcatgccgcgcgaggccatcaatatgggtggtgtctgcgaagtggtcgatcttagccaggtaagccagcaaatgttggcaaaaattagtgccggacaggcgatacgtatttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I13712_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgacttcatctctgccctgtgggcaaacgtctttattgttacagatgaccgagcgcctggcgctttccgacgcgcattttcggcggataagtcaattgatctatcaacgagccgggatcgttctggctgaccataaacgcgacatggtttacaaccgactggttcgtcgtttgcgttcgctgggactgacggatttcggtcattatctgaacttgctggaatctaatcagcacagcggtgagtggcaggcgtttatcaattcgctgaccacgaatctgacggcatttttccgtgaggcacatcatttccctctgctcgcggatcacgcacgtcgccgttctggcgagtatcgcgtatggagcgcggcggcttcgaccggcgaagagccgtacagcattgcgatgacgctggctgacacattgggcaccgcgcccggacgctggaaagtgtttgccagtgatatcgacaccgaagtgctggaaaaagccagaagcggtatctatcgccatgaagagttgaaaaacctgacgccgcagcaactgcaacggtatttcatgcgagggacggggccgcatgaagggctggtacgcgtgcgtcaggagctggcgaactatgttgattttgccccgctgaatctactggcgaaacagtacaccgtgccggggccgtttgatgcgatcttctgtcgtaacgtcatgatctacttcgatcaaactacccagcaggagattttgcgccgctttgttccgctccttaaacccgacggattgctgtttgcgggtcactctgaaaactttagccaccttgagcgccgcttcacgctgcgtggtcagacggtgtatgcgctaagtaaggattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I13730_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcaaaatcagggtgttatctgtcgatgattcggcactgatgcgccagatcatgacagaaatcatcaacagccatagcgacatggaaatggtggcgaccgcgcctgatccgctggtcgcgcgtgacttgattaagaaattcaatcccgatgtgctgacgctggatgttgaaatgccgcggatggacggactggatttcctcgaaaaattaatgcgtttgcgtccaatgcccgttgtgatggtttcttccctgaccggcaaagggtcagaagtcacgctgcgcgcgctggagctgggggcgatagattttgtcaccaaaccgcaactgggtattcgcgaaggtatgctggcgtataacgaaatgattgctgaaaaggtgcgtacggcagcaaaggcgagccttgcagcacataagccattgtcggcaccgacaacgctgaaggcggggccgttgttgagttctgaaaaactgattgcgattggtgcttcaacgggtggaactgaggcaattcgtcacgtactgcaaccgttgccgctttccagcccggcactgttaattacccagcatatgccgcccggtttcacccgctcttttgccgacagacttaataagctttgccagatcggggttaaagaagccgaagacggagaacgtgtcttgccggggcatgcctatattgcgccgggcgatcggcatatggagctgtcgcgtagtggcgcaaattaccaaatcaaaattcacgatggcccggcggttaaccgtcatcggccttcggtagatgtgttgttccattctgtcgccaaacaggcggggcgtaatgcggttggggtgatcctgaccggtatgggcaacgacggcgcggcgggaatgttggcgatgcgtcaggcgggggcatggacccttgcgcaaaacgaagcaagttgcgtggtgttcggcatgccgcgcgaggccatcaatatgggtggtgtctgcgaagtggtcgatcttagccaggtaagccagcaaatgttggcaaaaattagtgccggacaggcgatacgtatttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgacttcatctctgccctgtgggcaaacgtctttattgttacagatgaccgagcgcctggcgctttccgacgcgcattttcggcggataagtcaattgatctatcaacgagccgggatcgttctggctgaccataaacgcgacatggtttacaaccgactggttcgtcgtttgcgttcgctgggactgacggatttcggtcattatctgaacttgctggaatctaatcagcacagcggtgagtggcaggcgtttatcaattcgctgaccacgaatctgacggcatttttccgtgaggcacatcatttccctctgctcgcggatcacgcacgtcgccgttctggcgagtatcgcgtatggagcgcggcggcttcgaccggcgaagagccgtacagcattgcgatgacgctggctgacacattgggcaccgcgcccggacgctggaaagtgtttgccagtgatatcgacaccgaagtgctggaaaaagccagaagcggtatctatcgccatgaagagttgaaaaacctgacgccgcagcaactgcaacggtatttcatgcgagggacggggccgcatgaagggctggtacgcgtgcgtcaggagctggcgaactatgttgattttgccccgctgaatctactggcgaaacagtacaccgtgccggggccgtttgatgcgatcttctgtcgtaacgtcatgatctacttcgatcaaactacccagcaggagattttgcgccgctttgttccgctccttaaacccgacggattgctgtttgcgggtcactctgaaaactttagccaccttgagcgccgcttcacgctgcgtggtcagacggtgtatgcgctaagtaaggattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z