LOCUS       BBa_I13771              3549 bp    DNA     linear       19-May-2024
DEFINITION  F2621 + Codon-CBA
ACCESSION   BBa_I13771
VERSION     BBa_I13771.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1167..3549
                     /label=BBa_I13770
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagaggcta gctactatct ctcgagagat tagtaccttt
     1201 ggagatctac tagagaaaga ggagaaatac tagatggtga gcaaaggcga agaggttatt
     1261 aaggagttta tgcgtttcaa agtccgcatg gagggttcta tgaatgggca tgagtttgag
     1321 atcgaaggcg aaggagaggg tcggccgtat gaagggaccc agacggcgaa actgaaggta
     1381 actaaaggag gcccattacc tttcgcctgg gatatattga gtccccaatt tatgtacggt
     1441 tcaaaagcat atgtgaagca cccggctgac attccagatt acaaaaaact ttccttccct
     1501 gaaggcttta agtgggagag agttatgaac ttcgaggacg gtgggctcgt cacagtaacc
     1561 caggattcga gcctgcaaga cggaacgctg atctataaag tgaaaatgcg aggcactaat
     1621 tttccgcccg atggtccggt tatgcagaag aaaacaatgg gctgggaggc gtctaccgaa
     1681 cgtttatacc cacgggatgg tgtcttgaaa ggggagattc accaagccct taagctgaaa
     1741 gatggaggcc actatcttgt cgagttcaag acgatttaca tggcaaaaaa gccggtgcag
     1801 ttacccggct attactatgt ggacaccaaa cttgatatca ccagccataa cgaagattac
     1861 acgatagtag aacaatatga acgctcagaa gggcgccatc acctcttttt gggccacggt
     1921 acgggatcca ccggctcggg tagctctggg actgctagtt cagaggacaa taacatggcg
     1981 gtcatcaagg agtttatgcg gttcaaagtt cgtatggaag gctccatgaa tggtcatgag
     2041 tttgaaattg agggagaagg cgaaggtcgc ccctatgagg ggacacaaac cgccaaactt
     2101 aaggtaacga aaggaggccc gctgccattc gcatgggata tactctcgcc tcagtttatg
     2161 tacggtagca aagcttatgt gaagcacccg gcggatatcc cagactacaa aaaattatct
     2221 ttcccggaag ggtttaagtg ggagagagtc atgaacttcg aagatggcgg tctggttacc
     2281 gtgactcagg acagttcatt gcaagatgga acacttattt ataaagtaaa aatgcgaggc
     2341 acgaactttc cccctgatgg tccggtcatg cagaagaaaa ccatggggtg ggaagcctcc
     2401 actgagcgtc tgtatccacg cgacggcgtt ttaaaaggtg aaatccatca ggcactgaag
     2461 ctcaaagacg gtggacatta cttggtggag tttaaaacca tctatatggc taagaaacct
     2521 gttcaacttc cgggatacta ttacgtcgat actaagttgg acattacgtc acacaatgag
     2581 gactatacca tcgtggagca gtacgagaga tcggaagggc gtcaccattt atttttatat
     2641 ggcatggatg agctgtataa gtaatattat ttgtattgat ctggtgaatt cgcggccgct
     2701 tctagagtac tagtagcggc cgctgcagta ttatttgtat tgatctcctt actagagaaa
     2761 gaggagaaat actagatgag caaaggcgaa gagctgttta ccggtgtggt tccgatttta
     2821 gtcgaattgg atggggacgt aaatggacat aagttctctg tgagtggcga gggtgaaggt
     2881 gacgcgacgt atgggaaact cactctcaag tttatctgca caaccggtaa gttaccagtt
     2941 ccttggccca cgctggtcac tacattcacc tacggtgtac agtgttttgc ccgttatccg
     3001 gaccacatga aacaacatga tttctttaaa tcagcaatgc cagaaggcta cgtgcaggag
     3061 cgcacgatat tctttaagga cgatggtaac tataaaaccc gggctgaagt taagttcgag
     3121 ggcgatactt tagtcaatag aattgaattg aagggaatcg actttaaaga agatggcaac
     3181 attcttggtc acaaactgga gtacaattat aactcccata aggtgtacat cacagcggac
     3241 aaacaaaaaa atggcataaa ggtaaacttt aaaacgcgac acaatattga agatggttcg
     3301 gttcagctcg ccgaccatta tcagcaaaac acccctatcg gggatggacc ggttttgctc
     3361 cccgataatc actatctgag cactcagtct aaattgagta aggacccgaa cgagaaacgt
     3421 gatcatatgg tcctcttgga gttcgttaca gcagctggca ttacccacgg tatggacgaa
     3481 ctctacaaat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta
     3541 tttatgcat
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