LOCUS       BBa_I13930              1721 bp    DNA     linear       17-May-2024
DEFINITION  c1 Delay Oscillator Intermediate Construct (I13202.C0051.B0015)
ACCESSION   BBa_I13930
VERSION     BBa_I13930.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..666
                     /label=LuxI
     CDS             82..666
                     /label=BBa_C0161
     stem_loop       686..729
                     /label=stem_loop
     misc_feature    675..754
                     /label=BBa_B0010
     terminator      675..754
                     /label=BBa_B0010
     stem_loop       770..789
                     /label=T7 TE
     misc_feature    796..796
                     /label=stop
     polyA_site      790..803
                     /label=polya
     misc_feature    763..803
                     /label=BBa_B0012
     terminator      763..803
                     /label=BBa_B0012
     misc_feature    1..803
                     /label=BBa_I13202
     CDS             813..1520
                     /label=cI lambda
     misc_feature    1521..1553
                     /label=LVA
     misc_feature    1560..1584
                     /label=Help:Barcodes
     CDS             810..1584
                     /label=BBa_C0051
     stem_loop       1604..1647
                     /label=stem_loop
     misc_feature    1593..1672
                     /label=BBa_B0010
     terminator      1593..1672
                     /label=BBa_B0010
     stem_loop       1688..1707
                     /label=T7 TE
     misc_feature    1714..1714
                     /label=stop
     polyA_site      1708..1721
                     /label=polya
     misc_feature    1681..1721
                     /label=BBa_B0012
     terminator      1681..1721
                     /label=BBa_B0012
     terminator      1593..1721
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      721 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      781 gggtgggcct ttctgcgttt atatactaga tgagcacaaa aaagaaacca ttaacacaag
      841 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg
      901 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt
      961 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca
     1021 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag
     1081 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc
     1141 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg
     1201 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca
     1261 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc
     1321 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta
     1381 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt
     1441 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt
     1501 ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1561 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     1621 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1681 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//