LOCUS       BBa_I13940              2804 bp    DNA     linear       07-May-2024
DEFINITION  Exogenous LuxI Relaxation Oscillator Test Construct Intermediate 
            (I0500.C0261.E0434)
ACCESSION   BBa_I13940
VERSION     BBa_I13940.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     misc_feature    1816..1847
                     /label=LVA
     CDS             1237..1815
                     /label=luxI
     misc_feature    1237..1854
                     /label=BBa_C0061
     misc_feature    1855..1879
                     /label=Help:Barcodes
     CDS             1237..1854
                     /label=BBa_C0061
     misc_feature    1892..1895
                     /label=conserved
     RBS             1888..1899
                     /label=BBa_B0034
     misc_feature    2662..2667
                     /label=stop
     misc_feature    2623..2661
                     /label=AAV
     misc_feature    1906..2667
                     /label=BBa_E0034
     stem_loop       2687..2730
                     /label=stem_loop
     misc_feature    2676..2755
                     /label=BBa_B0010
     terminator      2676..2755
                     /label=BBa_B0010
     stem_loop       2771..2790
                     /label=T7 TE
     misc_feature    2797..2797
                     /label=stop
     polyA_site      2791..2804
                     /label=polya
     misc_feature    2764..2804
                     /label=BBa_B0012
     terminator      2764..2804
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ctataatgat aaaaaaatcg
     1261 gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg ttatcaagtg
     1321 tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc agatgagtat
     1381 gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt aagtggatgc
     1441 tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc tgaattgctt
     1501 ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt tgctgtaggt
     1561 aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact atttgaagct
     1621 atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac atcaacagca
     1681 atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga caaagaaatt
     1741 catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga acagtttaaa
     1801 aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta ataactctga
     1861 tagtgctagt gtagatctct actagagaaa gaggagaaat actagatggt gagcaagggc
     1921 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1981 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2041 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     2101 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc
     2161 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2221 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2281 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2341 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2401 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2461 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     2521 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2581 accgccgccg ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac
     2641 gaaaactacg ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc
     2701 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2761 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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