LOCUS       BBa_I13975              2239 bp    DNA     linear       08-May-2024
DEFINITION  intermediate for lac synchro device
ACCESSION   BBa_I13975
VERSION     BBa_I13975.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     misc_feature    1319..1335
                     /label=lac O1
     promoter        1336..1341
                     /label=-35
     misc_feature    1342..1358
                     /label=lac O1
     promoter        1359..1364
                     /label=-10
     misc_feature    1317..1370
                     /label=BBa_R0011
     promoter        1317..1370
                     /label=BBa_R0011
     misc_feature    1383..1386
                     /label=conserved
     RBS             1379..1390
                     /label=BBa_B0034
     misc_feature    1976..2007
                     /label=LVA
     CDS             1397..1975
                     /label=luxI
     misc_feature    1397..2014
                     /label=BBa_C0061
     misc_feature    2015..2039
                     /label=Help:Barcodes
     CDS             1397..2014
                     /label=BBa_C0061
     stem_loop       2059..2102
                     /label=stem_loop
     misc_feature    2048..2127
                     /label=BBa_B0010
     terminator      2048..2127
                     /label=BBa_B0010
     stem_loop       2143..2162
                     /label=T7 TE
     misc_feature    2169..2169
                     /label=stop
     polyA_site      2163..2176
                     /label=polya
     misc_feature    2136..2176
                     /label=BBa_B0012
     terminator      2136..2176
                     /label=BBa_B0012
     misc_feature    2185..2204
                     /label=LuxR/HSL
     promoter        2204..2209
                     /label=-35
     promoter        2226..2231
                     /label=-10
     misc_feature    2237..2237
                     /label=start
     misc_feature    2185..2239
                     /label=BBa_R0062
     promoter        2185..2239
                     /label=BBa_R0062
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt
     1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac tactagagaa
     1381 agaggagaaa tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc
     1441 ggaggagtat aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg
     1501 ggacttagtt gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata
     1561 tatttatgct tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac
     1621 aggtgattat atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa
     1681 agatcctaat atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa
     1741 taactctgct agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag
     1801 tcaaggtatt acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg
     1861 tattaaagtt ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa
     1921 atcggttgta ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaatgctgc
     1981 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct
     2041 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2101 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     2161 cctttctgcg tttatatact agagacctgt aggatcgtac aggtttacgc aagaaaatgg
     2221 tttgttatag tcgaataaa
//