LOCUS       BBa_I14021               810 bp    DNA     linear       15-May-2024
DEFINITION  plTetO1 . RBS . CinI
ACCESSION   BBa_I14021
VERSION     BBa_I14021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..746
                     /label=CinI
     misc_feature    747..779
                     /label=LVA
     misc_feature    786..810
                     /label=Help:Barcodes
     CDS             84..785
                     /label=BBa_C0076
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgttcg ttatcatcca ggctcacgaa taccagaaat
      121 acgctgctgt tctggaccag atgttccgtc tgcgtaaaaa agttttcgct gacaccctgt
      181 gctgggacgt tccggttatc ggtccgtacg aacgtgactc ctacgactcc ctggctccgg
      241 cttacctggt ttggtgcaac gactcccgta cccgtctgta cggtggtatg cgtctgatgc
      301 cgaccaccgg tccgaccctg ctgtacgacg ttttccgtga aaccttcccg gacgctgctg
      361 acctgatcgc tccgggtatc tgggaaggta cccgtatgtg catcgacgaa gaagctatcg
      421 ctaaagactt cccggaaatc gacgctggtc gtgctttctc catgatgctg ctggctctgt
      481 gcgaatgcgc tctggaccac ggtatccaca ccatgatctc caactacgaa ccgtacctga
      541 aacgtgttta caaacgtgct ggtgctgaag ttgaagaact gggtcgtgct gacggttacg
      601 gtaaataccc ggtttgctgc ggtgctttcg aagtttccga ccgtgttctg cgtaaaatgc
      661 gtgctgctct gggtctgacc ctgccgctgt acgttcgtca cgttccggct cgttccgttg
      721 ttacccagtt cctggaaatg gctgctgctg ctaacgacga aaactacgct ctggttgctt
      781 aataaccctg atagtgctag tgtagatccc
//