LOCUS       BBa_I14047              3118 bp    DNA     linear       13-May-2024
DEFINITION  LacI toggle
ACCESSION   BBa_I14047
VERSION     BBa_I14047.1  
FEATURES             Location/Qualifiers
     misc_feature    1..77
                     /label=PenI
     promoter        23..28
                     /label=-35
     promoter        46..51
                     /label=-10
     misc_feature    31..53
                     /label=dimer left half
     misc_feature    55..76
                     /label=dimer right half
     promoter        1..77
                     /label=BBa_R0074
     RBS             86..98
                     /label=RBS-3\Weak
     RBS             92..95
                     /label=RBS
     misc_feature    86..98
                     /label=BBa_B0032
     RBS             86..98
                     /label=BBa_B0032
     misc_feature    1194..1232
                     /label=LVA
     CDS             105..1232
                     /label=lacI-LVA
     misc_feature    105..1232
                     /label=BBa_C0012
     misc_feature    1233..1257
                     /label=Help:Barcodes
     CDS             105..1232
                     /label=BBa_C0012
     RBS             1266..1278
                     /label=RBS-3\Weak
     RBS             1272..1275
                     /label=RBS
     misc_feature    1266..1278
                     /label=BBa_B0032
     RBS             1266..1278
                     /label=BBa_B0032
     CDS             1285..2160
                     /label=araC
     misc_feature    2161..2193
                     /label=LVA
     misc_feature    2200..2224
                     /label=Help:Barcodes
     CDS             1285..2199
                     /label=BBa_C0080
     RBS             2233..2247
                     /label=RBS-1\Strong
     RBS             2240..2244
                     /label=RBS
     misc_feature    2233..2247
                     /label=BBa_B0030
     RBS             2233..2247
                     /label=BBa_B0030
     CDS             2254..3015
                     /label=YFP (LVA)
     misc_feature    3010..3015
                     /label=2
     misc_feature    2971..3009
                     /label=SsrA
     misc_feature    2495..2495
                     /label=A (Q->L)
     misc_feature    2254..3015
                     /label=BBa_E0032
     misc_feature    2254..3015
                     /label=BBa_E0032
     stem_loop       3031..3050
                     /label=T7 TE
     misc_feature    3057..3057
                     /label=stop
     polyA_site      3051..3064
                     /label=polya
     misc_feature    3024..3064
                     /label=BBa_B0012
     terminator      3024..3064
                     /label=BBa_B0012
     stem_loop       3085..3107
                     /label=stem_loop
     misc_feature    3073..3118
                     /label=BBa_B0011
     terminator      3073..3118
                     /label=BBa_B0011
ORIGIN
        1 tcatttccaa ccgaaaaaag gcttgcattt aaatcttaca tatgtaatac tttcaaagac
       61 tacatttgta agatttgtac tagagtcaca caggaaagta ctagatggtg aatgtgaaac
      121 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
      181 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
      241 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
      301 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
      361 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
      421 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
      481 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
      541 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
      601 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
      661 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
      721 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
      781 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
      841 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
      901 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
      961 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     1021 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
     1081 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
     1141 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa
     1201 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac
     1261 tagagtcaca caggaaagta ctagatggct gaagcgcaaa atgatcccct gctgccggga
     1321 tactcgttta acgcccatct ggtggcgggt ttaacgccga ttgaggccaa tggttatctc
     1381 gattttttta tcgaccgacc gctgggaatg aaaggttata ttctcaatct caccattcgc
     1441 ggtcaggggg tggtgaaaaa tcagggacga gaatttgtct gccgaccggg tgatattttg
     1501 ctgttcccgc caggagagat tcatcactac ggtcgtcatc cggaggctcg cgaatggtat
     1561 caccagtggg tttactttcg tccgcgcgcc tactggcatg aatggcttaa ctggccgtca
     1621 atatttgcca atacgggttt ctttcgcccg gatgaagcgc accagccgca tttcagcgac
     1681 ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc gctattcgga gctgctggcg
     1741 ataaatctgc ttgagcaatt gttactgcgg cgcatggaag cgattaacga gtcgctccat
     1801 ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca tcagcgatca cctggcagac
     1861 agcaattttg atatcgccag cgtcgcacag catgtttgcc tgtcgccgtc gcgtctgtca
     1921 catcttttcc gccagcagtt agggattagc gtcttaagct ggcgcgagga ccaacgcatc
     1981 agccaggcga agctgctttt gagcactacc cggatgccta tcgccaccgt cggtcgcaat
     2041 gttggttttg acgatcaact ctatttctcg cgagtattta aaaaatgcac cggggccagc
     2101 ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg atgtagccgt caagttgtca
     2161 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga
     2221 tcactactag agattaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc
     2281 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2341 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2401 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     2461 caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg
     2521 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2581 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2641 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2701 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2761 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2821 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     2881 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2941 atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct
     3001 ttagtagctt aataatacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     3061 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattattt
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