LOCUS       BBa_I14053              1232 bp    DNA     linear       14-May-2024
DEFINITION  C4, LacI Router (weak RBS)
ACCESSION   BBa_I14053
VERSION     BBa_I14053.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     misc_feature    1..51
                     /label=modified P(Rhl)
     promoter        21..50
                     /label=-35
     promoter        1..51
                     /label=BBa_I14017
     misc_feature    66..69
                     /label=conserved
     RBS             60..73
                     /label=BBa_B0031
     misc_feature    1169..1207
                     /label=LVA
     CDS             80..1207
                     /label=lacI-LVA
     misc_feature    80..1207
                     /label=BBa_C0012
     misc_feature    1208..1232
                     /label=Help:Barcodes
     CDS             80..1207
                     /label=BBa_C0012
ORIGIN
        1 tcctgcatga attggtaggc ttacgcaaga aaatggtttg ttatagtcga atactagagt
       61 cacacaggaa acctactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
      121 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      181 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
      241 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
      301 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
      361 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
      421 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
      481 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
      541 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
      601 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
      661 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
      721 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
      781 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
      841 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
      901 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
      961 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     1021 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     1081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1141 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     1201 ttaataactc tgatagtgct agtgtagatc tc
//