LOCUS       BBa_I14056              2827 bp    DNA     linear       13-May-2024
DEFINITION  Tet Toggle (strong RBS, weaker RBS)
ACCESSION   BBa_I14056
VERSION     BBa_I14056.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    1173..1211
                     /label=LVA
     CDS             84..1211
                     /label=lacI-LVA
     misc_feature    84..1211
                     /label=BBa_C0012
     misc_feature    1212..1236
                     /label=Help:Barcodes
     CDS             84..1211
                     /label=BBa_C0012
     RBS             1245..1255
                     /label=RBS-4\Weaker
     RBS             1251..1254
                     /label=RBS
     misc_feature    1245..1255
                     /label=BBa_B0033
     RBS             1245..1255
                     /label=BBa_B0033
     CDS             1265..1972
                     /label=cI lambda
     misc_feature    1973..2005
                     /label=LVA
     misc_feature    2012..2036
                     /label=Help:Barcodes
     CDS             1262..2011
                     /label=BBa_C0051
     RBS             2045..2059
                     /label=RBS-1\Strong
     RBS             2052..2056
                     /label=RBS
     misc_feature    2045..2059
                     /label=BBa_B0030
     RBS             2045..2059
                     /label=BBa_B0030
     CDS             2066..2827
                     /label=YFP (LVA)
     misc_feature    2822..2827
                     /label=2
     misc_feature    2783..2821
                     /label=SsrA
     misc_feature    2307..2307
                     /label=A (Q->L)
     misc_feature    2066..2827
                     /label=BBa_E0032
     misc_feature    2066..2827
                     /label=BBa_E0032
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg
      121 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
      181 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
      241 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
      301 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
      361 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
      421 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
      481 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
      541 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
      601 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
      661 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
      721 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
      781 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
      841 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
      901 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
      961 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     1021 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     1081 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1141 cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag
     1201 tagcttaata actctgatag tgctagtgta gatctctact agagtcacac aggactacta
     1261 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
     1321 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
     1381 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
     1441 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
     1501 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
     1561 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
     1621 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
     1681 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
     1741 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
     1801 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
     1861 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
     1921 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa
     1981 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact
     2041 agagattaaa gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt
     2101 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2161 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2221 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt
     2281 cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg
     2341 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2401 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2461 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2521 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2581 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2641 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     2701 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2761 cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc
     2821 ttaataa
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