LOCUS BBa_I20243 3920 bp DNA linear 13-May-2024 DEFINITION Autoregulated synthesis of T7 RNAP with lacI negative feedback ACCESSION BBa_I20243 VERSION BBa_I20243.1 FEATURES Location/Qualifiers promoter 1..20 /label=BBa_R0183 fragment misc_feature 20..44 /label=lacI binding site misc_feature 31..31 /label=Center of binding site symmetry promoter 1..44 /label=BBa_R0184 RBS 53..65 /label=RBS-3\Weak RBS 59..62 /label=RBS misc_feature 53..65 /label=BBa_B0032 RBS 53..65 /label=BBa_B0032 misc_feature 1158..1182 /label=Help:Barcodes CDS 72..1182 /label=BBa_C0013 misc_feature 53..1182 /label=BBa_S03690 RBS 1191..1203 /label=RBS-3\Weak RBS 1197..1200 /label=RBS misc_feature 1191..1203 /label=BBa_B0032 RBS 1191..1203 /label=BBa_B0032 misc_feature 53..1203 /label=BBa_S03746 promoter 3707..3734 /label=putative E. coli promoter CDS 1210..3864 /label=BBa_I2032 terminator 3873..3920 /label=BBa_B0016 misc_feature 1210..3920 /label=BBa_S03693 misc_feature 53..3920 /label=BBa_I20241 ORIGIN 1 tcatacgact cactataggg gaattgtgag cggataacaa ttcctactag agtcacacag 61 gaaagtacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 121 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 181 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 241 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 301 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 361 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 421 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 481 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 541 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 601 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 661 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 721 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 781 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 841 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg 901 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 961 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 1021 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 1081 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 1141 aaagcgggca gtaataacac tgatagtgct agtgtagatc actactagag tcacacagga 1201 aagtactaga tgaacacgat taacatcgct aagaacgact tctctgacat cgaactggct 1261 gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg 1321 gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt 1381 caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta 1441 ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc 1501 aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc 1561 accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta 1621 gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt 1681 gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc 1741 tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt 1801 ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc 1861 gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta 1921 ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt 1981 gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg 2041 tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg 2101 cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac 2161 aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc 2221 gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgagcgt 2281 gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg 2341 aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc 2401 cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct 2461 tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac 2521 gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac 2581 tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag 2641 cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg 2701 gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag 2761 tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac 2821 gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc 2881 gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa 2941 gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg 3001 accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct 3061 ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg 3121 gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca 3181 gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc tggatacatg 3241 gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac 3301 tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag 3361 attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag 3421 gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta 3481 cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt 3541 atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg 3601 gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt cggtaccatt 3661 ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag 3721 tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa 3781 ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag 3841 tcggacttcg cgttcgcgta ataatactag agctagcata accccttggg gcctctaaac 3901 gggtcttgag gggttttttg //