LOCUS       BBa_I20253              2726 bp    DNA     linear       29-Apr-2024
DEFINITION  Protein Generator for T7 RNAP
ACCESSION   BBa_I20253
VERSION     BBa_I20253.1  
FEATURES             Location/Qualifiers
     RBS             1..9
                     /label=BBa_B0073
     promoter        2513..2540
                     /label=putative E. coli promoter
     CDS             16..2670
                     /label=BBa_I2032
     terminator      2679..2726
                     /label=BBa_B0016
ORIGIN
        1 tcacaccact actagatgaa cacgattaac atcgctaaga acgacttctc tgacatcgaa
       61 ctggctgcta tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa
      121 cagttggccc ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt
      181 gagcgtcaac ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact
      241 accctactcc ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag
      301 cgcggcaagc gcccgacagc cttccagttc ctgcaagaaa tcaagccgga agccgtagcg
      361 tacatcacca ttaagaccac tctggcttgc ctaaccagtg ctgacaatac aaccgttcag
      421 gctgtagcaa gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt
      481 gaccttgaag ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg
      541 cacgtctaca agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta
      601 ctcggtggcg aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc
      661 tgcatcgaga tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc
      721 gtagtaggtc aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca
      781 acccgtgcag gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct
      841 aagccgtgga ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg
      901 ctggtgcgta ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag
      961 gtgtacaaag cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta
     1021 gcggtcgcca acgtaatcac caagtggaag cattgtccgg tcgaggacat ccctgcgatt
     1081 gagcgtgaag aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc
     1141 gcgtggaaac gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt
     1201 atcagccttg agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg
     1261 ttcccttaca acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa
     1321 ggtaacgata tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa
     1381 ggttactact ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc
     1441 cctgagcgca tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct
     1501 ccactggaga acacttggtg ggctgagcaa gattctccgt tctgcttcct tgcgttctgc
     1561 tttgagtacg ctggggtaca gcaccacggc ctgagctata actgctccct tccgctggcg
     1621 tttgacgggt cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt
     1681 ggtcgcgcgg ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct
     1741 aagaaagtca acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt
     1801 accgtgaccg atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca
     1861 ctggctggtc aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg
     1921 acgctggctt acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt
     1981 cagccagcta ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga
     2041 tacatggcta agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca
     2101 atgaactggc ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact
     2161 ggagagattc ttcgcaagcg ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg
     2221 tggcaggaat acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc
     2281 cgcttacagc ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag
     2341 tctggtatcg ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta
     2401 gtgtgggcac acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt
     2461 accattccgg ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca
     2521 tatgagtctt gtgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag
     2581 tctcaattgg acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc
     2641 ttagagtcgg acttcgcgtt cgcgtaataa tactagagct agcataaccc cttggggcct
     2701 ctaaacgggt cttgaggggt tttttg
//