LOCUS BBa_I20257 3912 bp DNA linear 12-May-2024 DEFINITION Autoregulated synthesis of T7 RNAP with lacI negative feedback (orthogonal translation) ACCESSION BBa_I20257 VERSION BBa_I20257.1 FEATURES Location/Qualifiers promoter 1..20 /label=BBa_R0183 fragment misc_feature 20..44 /label=lacI binding site misc_feature 31..31 /label=Center of binding site symmetry promoter 1..44 /label=BBa_R0184 RBS 53..61 /label=BBa_B0073 misc_feature 1154..1178 /label=Help:Barcodes CDS 68..1178 /label=BBa_C0013 misc_feature 53..1178 /label=BBa_S03910 misc_feature 1..1178 /label=BBa_S03914 RBS 1187..1195 /label=BBa_B0073 promoter 3699..3726 /label=putative E. coli promoter CDS 1202..3856 /label=BBa_I2032 terminator 3865..3912 /label=BBa_B0016 misc_feature 1187..3912 /label=BBa_I20253 ORIGIN 1 tcatacgact cactataggg gaattgtgag cggataacaa ttcctactag agtcacacca 61 ctactagatg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca 121 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt 181 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg 241 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca 301 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat 361 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg 421 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc 481 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag 541 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg 601 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct 661 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg 721 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt 781 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac 841 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga 901 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca 961 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct 1021 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc 1081 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 1141 cgggcagtaa taacactgat agtgctagtg tagatcacta ctagagtcac accactacta 1201 gatgaacacg attaacatcg ctaagaacga cttctctgac atcgaactgg ctgctatccc 1261 gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt tggcccttga 1321 gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa 1381 agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc tactccctaa 1441 gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc 1501 gacagccttc cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca tcaccattaa 1561 gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg tagcaagcgc 1621 aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa 1681 gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg tctacaagaa 1741 agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg gtggcgaggc 1801 gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca tcgagatgct 1861 cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag taggtcaaga 1921 ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc 1981 gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg 2041 cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca 2101 cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt acaaagcgat 2161 taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg tcgccaacgt 2221 aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc gtgaagaact 2281 cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc 2341 tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca gccttgagtt 2401 catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc cttacaacat 2461 ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta acgatatgac 2521 caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt actactggct 2581 gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa 2641 gttcattgag gaaaaccacg agaacatcat ggcttgcgct aagtctccac tggagaacac 2701 ttggtgggct gagcaagatt ctccgttctg cttccttgcg ttctgctttg agtacgctgg 2761 ggtacagcac cacggcctga gctataactg ctcccttccg ctggcgtttg acgggtcttg 2821 ctctggcatc cagcacttct ccgcgatgct ccgagatgag gtaggtggtc gcgcggttaa 2881 cttgcttcct agtgaaaccg ttcaggacat ctacgggatt gttgctaaga aagtcaacga 2941 gattctacaa gcagacgcaa tcaatgggac cgataacgaa gtagttaccg tgaccgatga 3001 gaacactggt gaaatctctg agaaagtcaa gctgggcact aaggcactgg ctggtcaatg 3061 gctggcttac ggtgttactc gcagtgtgac taagcgttca gtcatgacgc tggcttacgg 3121 gtccaaagag ttcggcttcc gtcaacaagt gctggaagat accattcagc cagctattga 3181 ttccggcaag ggtctgatgt tcactcagcc gaatcaggct gctggataca tggctaagct 3241 gatttgggaa tctgtgagcg tgacggtggt agctgcggtt gaagcaatga actggcttaa 3301 gtctgctgct aagctgctgg ctgctgaggt caaagataag aagactggag agattcttcg 3361 caagcgttgc gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc aggaatacaa 3421 gaagcctatt cagacgcgct tgaacctgat gttcctcggt cagttccgct tacagcctac 3481 cattaacacc aacaaagata gcgagattga tgcacacaaa caggagtctg gtatcgctcc 3541 taactttgta cacagccaag acggtagcca ccttcgtaag actgtagtgt gggcacacga 3601 gaagtacgga atcgaatctt ttgcactgat tcacgactcc ttcggtacca ttccggctga 3661 cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt gacacatatg agtcttgtga 3721 tgtactggct gatttctacg accagttcgc tgaccagttg cacgagtctc aattggacaa 3781 aatgccagca cttccggcta aaggtaactt gaacctccgt gacatcttag agtcggactt 3841 cgcgttcgcg taataatact agagctagca taaccccttg gggcctctaa acgggtcttg 3901 aggggttttt tg //