LOCUS       BBa_I20273              2811 bp    DNA     linear       11-May-2024
DEFINITION  T7 RNAP generator
ACCESSION   BBa_I20273
VERSION     BBa_I20273.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     promoter        2517..2544
                     /label=putative E. coli promoter
     CDS             20..2674
                     /label=BBa_I2032
     stem_loop       2694..2737
                     /label=stem_loop
     misc_feature    2683..2762
                     /label=BBa_B0010
     terminator      2683..2762
                     /label=BBa_B0010
     stem_loop       2778..2797
                     /label=T7 TE
     misc_feature    2804..2804
                     /label=stop
     polyA_site      2798..2811
                     /label=polya
     misc_feature    2771..2811
                     /label=BBa_B0012
     terminator      2771..2811
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgaacacgat taacatcgct aagaacgact tctctgacat
       61 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg
      121 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat
      181 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat
      241 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc
      301 taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt
      361 agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt
      421 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat
      481 ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt
      541 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg
      601 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt
      661 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc
      721 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat
      781 cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc
      841 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct
      901 ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc
      961 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt
     1021 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc
     1081 gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct
     1141 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg
     1201 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat
     1261 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc
     1321 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa
     1381 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc
     1441 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa
     1501 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt
     1561 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct
     1621 ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt
     1681 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt
     1741 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt
     1801 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa
     1861 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt
     1921 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac
     1981 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc
     2041 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga
     2101 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa
     2161 gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc
     2221 tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca
     2281 gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca
     2341 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac
     2401 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt
     2461 cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga
     2521 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca
     2581 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga
     2641 catcttagag tcggacttcg cgttcgcgta ataatactag agccaggcat caaataaaac
     2701 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2761 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//