LOCUS       BBa_I2028               1828 bp    DNA     linear       17-May-2024
DEFINITION  RhlR Receiver Test (R1075.B0034.C0071.B0015.R0071.E0022)
ACCESSION   BBa_I2028
VERSION     BBa_I2028.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..798
                     /label=rhlR
     misc_feature    799..831
                     /label=LVA
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             76..837
                     /label=BBa_C0071
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=RhlR
     promoter        1026..1031
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     promoter        1008..1060
                     /label=BBa_R0071
     CDS             1067..1828
                     /label=CFP (LVA)
     misc_feature    1785..1822
                     /label=SsrA
     misc_feature    1823..1828
                     /label=2
     misc_feature    1135..1135
                     /label=A
     misc_feature    1067..1828
                     /label=BBa_E0022
     misc_feature    1067..1828
                     /label=BBa_E0022
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatgag gaatgacgga ggctttttgc tgtggtggga cggtttgcgt
      121 agcgagatgc agccgatcca cgacagccag ggcgtgttcg ccgtcctgga aaaggaagtg
      181 cggcgcctgg gcttcgatta ctacgcctat ggcgtgcgcc acacgattcc cttcacccgg
      241 ccgaagaccg aggtccatgg cacctatccc aaggcctggc tggagcgata ccagatgcag
      301 aactacgggg ccgtggatcc ggcgatcctc aacggcctgc gctcctcgga aatggtggtc
      361 tggagcgaca gcctgttcga ccagagccgg atgctctgga acgaggctcg cgattggggc
      421 ctctgtgtcg gcgcgacctt gccgatccgc gcgccgaaca atttgctcag cgtgctttcc
      481 gtggcgcgcg accagcagaa catctccagc ttcgagcgcg aggaaatccg cctgcggctg
      541 cgttgcatga tcgagttgct gacccagaag ctgaccgacc tggagcatcc gatgctgatg
      601 tccaacccgg tctgcctgag ccatcgcgaa cgcgagatcc tgcaatggac cgccgacggc
      661 aagagttccg gggaaatcgc catcatcctg agcatctccg agagcacggt gaacttccac
      721 cacaagaaca tccagaagaa gttcgacgcg ccgaacaaga cgctggctgc cgcctacgcc
      781 gcggcgctgg gtctcatcgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc tgtgaaatct
     1021 ggcagttacc gttagctttc gaattggcta aaaagtgttc tactagatgg tgagcaaggg
     1081 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg
     1141 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1201 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1261 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1321 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1381 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1441 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1501 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     1561 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1621 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1681 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1741 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggcctg ctgcaaacga
     1801 cgaaaactac gctttagtag cttaataa
//