LOCUS       BBa_I20283              1803 bp    DNA     linear       17-May-2024
DEFINITION  Adaptable Vaccine Antigen Secretor
ACCESSION   BBa_I20283
VERSION     BBa_I20283.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     RBS             32..44
                     /label=RBS-3\Weak
     RBS             38..41
                     /label=RBS
     misc_feature    32..44
                     /label=BBa_B0032
     RBS             32..44
                     /label=BBa_B0032
     CDS             54..670
                     /label=tetR
     misc_feature    671..704
                     /label=SsrA
     misc_feature    711..735
                     /label=Help:Barcodes
     CDS             51..735
                     /label=BBa_C0040
     misc_feature    744..777
                     /label=B1002
     stem_loop       753..768
                     /label=stem loop
     polyA_site      768..774
                     /label=Poly A tail
     polyA_site      747..752
                     /label=Poly A tail
     terminator      744..777
                     /label=BBa_B1002
     misc_feature    786..804
                     /label=TetR 1
     misc_feature    786..839
                     /label=BBa_R0040
     promoter        805..810
                     /label=-35
     misc_feature    811..829
                     /label=TetR 2
     promoter        828..833
                     /label=-10
     promoter        786..839
                     /label=BBa_R0040
     RBS             848..860
                     /label=RBS-3\Weak
     RBS             854..857
                     /label=RBS
     misc_feature    848..860
                     /label=BBa_B0032
     RBS             848..860
                     /label=BBa_B0032
     CDS             870..1577
                     /label=cI lambda
     misc_feature    1578..1610
                     /label=LVA
     misc_feature    1617..1641
                     /label=Help:Barcodes
     CDS             867..1641
                     /label=BBa_C0051
     misc_feature    1650..1683
                     /label=B1002
     stem_loop       1659..1674
                     /label=stem loop
     polyA_site      1674..1680
                     /label=Poly A tail
     polyA_site      1653..1658
                     /label=Poly A tail
     terminator      1650..1683
                     /label=BBa_B1002
     promoter        1729..1734
                     /label=-10
     promoter        1706..1711
                     /label=-35
     misc_feature    1716..1732
                     /label=OR1
     misc_feature    1692..1708
                     /label=OR2
     misc_feature    1692..1740
                     /label=BBa_R0051
     promoter        1692..1740
                     /label=BBa_R0051
     RBS             1749..1761
                     /label=RBS-3\Weak
     RBS             1755..1758
                     /label=RBS
     misc_feature    1749..1761
                     /label=BBa_B0032
     RBS             1749..1761
                     /label=BBa_B0032
     misc_feature    1770..1803
                     /label=B1002
     stem_loop       1779..1794
                     /label=stem loop
     polyA_site      1794..1800
                     /label=Poly A tail
     polyA_site      1773..1778
                     /label=Poly A tail
     terminator      1770..1803
                     /label=BBa_B1002
ORIGIN
        1 taatacgact cactataggg agatactaga gtcacacagg aaagtactag atgtccagat
       61 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
      121 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
      181 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
      241 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
      301 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
      361 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
      421 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
      481 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
      541 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
      601 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
      661 aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt
      721 gctagtgtag atcactacta gagcgcaaaa aaccccgctt cggcggggtt ttttcgctac
      781 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact
      841 actagagtca cacaggaaag tactagatga gcacaaaaaa gaaaccatta acacaagagc
      901 agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct
      961 tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat
     1021 ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa attctcaaag
     1081 ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg
     1141 ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg
     1201 cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa
     1261 gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga
     1321 ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg
     1381 agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct
     1441 tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc
     1501 caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc
     1561 ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct taataacgct
     1621 gatagtgcta gtgtagatcg ctactagagc gcaaaaaacc ccgcttcggc ggggtttttt
     1681 cgctactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc
     1741 tactagagtc acacaggaaa gtactagagc gcaaaaaacc ccgcttcggc ggggtttttt
     1801 cgc
//