LOCUS       BBa_I2038               2055 bp    DNA     linear       02-May-2024
DEFINITION  mCherry reporter device and GFP reporter device in sequence
ACCESSION   BBa_I2038
VERSION     BBa_I2038.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     stem_loop       56..118
                     /label=hp1
     misc_feature    119..129
                     /label=ss1
     stem_loop       130..158
                     /label=hp2
     misc_feature    159..170
                     /label=ss2
     misc_feature    56..170
                     /label=BBa_B0100
     misc_feature    171..176
                     /label=AvrII/XbaI mixed site
     misc_feature    171..176
                     /label=BBa_B0104
     misc_feature    1..176
                     /label=BBa_S00164
     RBS             177..196
                     /label=BBa_B2040
     CDS             197..907
                     /label=mCherry
     misc_feature    548..548
                     /label=C->T (removing PstI site)
     misc_feature    197..910
                     /label=BBa_J06504
     stem_loop       924..976
                     /label=stem_loop
     misc_feature    919..976
                     /label=BBa_B0101
     stem_loop       996..1039
                     /label=stem_loop
     misc_feature    985..1064
                     /label=BBa_B0010
     terminator      985..1064
                     /label=BBa_B0010
     stem_loop       1080..1099
                     /label=T7 TE
     misc_feature    1106..1106
                     /label=stop
     polyA_site      1100..1113
                     /label=polya
     misc_feature    1073..1113
                     /label=BBa_B0012
     terminator      1073..1113
                     /label=BBa_B0012
     misc_feature    197..1113
                     /label=BBa_S00162
     misc_feature    1..1113
                     /label=BBa_I20240
     misc_feature    1122..1140
                     /label=TetR 1
     misc_feature    1122..1175
                     /label=BBa_R0040
     promoter        1141..1146
                     /label=-35
     misc_feature    1147..1165
                     /label=TetR 2
     promoter        1164..1169
                     /label=-10
     promoter        1122..1175
                     /label=BBa_R0040
     RBS             1184..1192
                     /label=BBa_B0073
     CDS             1199..1918
                     /label=GFP protein
     CDS             1199..1918
                     /label=BBa_E0040
     stem_loop       1938..1981
                     /label=stem_loop
     misc_feature    1927..2006
                     /label=BBa_B0010
     terminator      1927..2006
                     /label=BBa_B0010
     stem_loop       2022..2041
                     /label=T7 TE
     misc_feature    2048..2048
                     /label=stop
     polyA_site      2042..2055
                     /label=polya
     misc_feature    2015..2055
                     /label=BBa_B0012
     terminator      2015..2055
                     /label=BBa_B0012
     misc_feature    1122..2055
                     /label=BBa_E70202
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacagccag
       61 gggtgctcgg cataagccga agatatcggt agagttaata ttgagcagat cccccggtga
      121 aggatttaac cgtgttatct cgttggagat attcatggcg tattttggat cctagaaaga
      181 catggagaca catttaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
      241 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      301 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      361 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      421 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      481 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      541 ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      601 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      661 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
      721 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      781 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      841 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      901 caagtaataa tactagagga tcgcctgatc ccggtgcacc cgggcagctg catagtctgg
      961 gtgcaccggg atcaggtact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1021 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1081 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga
     1141 ttgacatccc tatcagtgat agagatactg agcactacta gagtcacacc actactagat
     1201 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1261 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1321 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1381 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     1441 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     1501 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     1561 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     1621 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     1681 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     1741 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     1801 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     1861 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
     1921 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1981 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2041 ctttctgcgt ttata
//