LOCUS       BBa_I2040               1248 bp    DNA     linear       12-May-2024
DEFINITION  lacI repressor expression device
ACCESSION   BBa_I2040
VERSION     BBa_I2040.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    1..75
                     /label=BBa_R4032
     misc_feature    1168..1192
                     /label=Help:Barcodes
     CDS             82..1192
                     /label=BBa_C0013
     terminator      1201..1248
                     /label=BBa_B0016
     misc_feature    82..1248
                     /label=BBa_S03691
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc
      121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
      181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
      241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
      301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
      361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
      421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
      481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
      541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
      601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
      661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
      721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
      781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
      841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
      901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
      961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1141 tcccgactgg aaagcgggca gtaataacac tgatagtgct agtgtagatc actactagag
     1201 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttg
//